PDF(Pubmed)
Abstract：
This study aimed to investigate the therapeutic potential of Citrullus mucosospermus extract (CME) in counteracting adipogenesis and its associated metabolic disturbances in murine models. In vitro experiments utilizing 3T3-L1 preadipocytes revealed that CME potently inhibited adipocyte differentiation, as evidenced by a dose-dependent reduction in lipid droplet formation. Remarkably, CME also attenuated glucose uptake and intracellular triglyceride accumulation in fully differentiated adipocytes, suggesting its ability to modulate metabolic pathways in mature adipose cells. Translating these findings to an in vivo setting, we evaluated the effects of CME in C57BL/6N mice fed a high-fat diet (HFD) for 10 weeks. CME administration, concomitantly with the HFD, resulted in a significant attenuation of body weight gain compared to the HFD control group. Furthermore, CME treatment led to substantial reductions in liver weight, total fat mass, and deposits of visceral and retroperitoneal adipose tissue, underscoring its targeted impact on adipose expansion. Histological analyses revealed the remarkable effects of CME on hepatic steatosis. While the HFD group exhibited severe lipid accumulation within liver lobules, CME dose-dependently mitigated this pathology, with the highest dose virtually abolishing hepatic fat deposition. An examination of adipose tissue revealed a progressive reduction in adipocyte hypertrophy upon CME treatment, culminating in a near-normalization of adipocyte morphology at the highest dose. Notably, CME exhibited potent anti-inflammatory properties, significantly attenuating the upregulation of pro-inflammatory cytokines\' mRNA levels (TNF-α, IL-1β and IL-6) in the livers of HFD-fed mice. This suggests a potential mechanism through which CME may exert protective effects against inflammation associated with obesity and fatty liver disease.
摘要：
这项研究旨在研究在小鼠模型中对抗脂肪形成及其相关代谢紊乱的治疗潜力。利用3T3-L1前脂肪细胞的体外实验表明，CME有效抑制脂肪细胞分化，脂滴形成的剂量依赖性减少证明了这一点。值得注意的是,CME还减弱了完全分化脂肪细胞的葡萄糖摄取和细胞内甘油三酯积累，表明其调节成熟脂肪细胞代谢途径的能力。将这些发现转化为体内环境，我们评估了CME在高脂饮食（HFD）喂养10周的C57BL/6N小鼠中的作用。CME管理部门,伴随着HFD，与HFD对照组相比,导致体重增加的显著衰减。此外，CME治疗导致肝脏重量大幅减少，总脂肪量,内脏和腹膜后脂肪组织的沉积，强调其对脂肪扩张的针对性影响。组织学分析显示CME对肝脂肪变性的显着影响。而HFD组肝小叶内表现出严重的脂质积累，CME剂量依赖性地减轻了这种病理，最高剂量几乎消除了肝脏脂肪沉积。脂肪组织检查显示CME治疗后脂肪细胞肥大逐渐减少，最高剂量时脂肪细胞形态接近正常化。值得注意的是,CME表现出有效的抗炎特性，显著减弱促炎细胞因子mRNA水平的上调(TNF-α，HFD喂养小鼠肝脏中的IL-1β和IL-6）。这表明CME可能通过其发挥针对与肥胖和脂肪肝疾病相关的炎症的保护作用的潜在机制。
