PDF(Pubmed)
Abstract：
Background: Hypertension poses a significant global health burden and is associated with cardiovascular morbidity. Chios mastic gum (CMG), derived from Pistacia lentiscus var. Chia, shows potential as a phytotherapeutic agent, due to its multifaceted beneficial effects. However, its anti-hypertensive effects and vascular, circulatory, and renal-related dysfunction, have not been thoroughly investigated. Herein, we aimed to explore the antihypertensive potential of CMG, focusing on vascular and renal endothelium, in vivo. Methods: Two models of hypertension in male rats, induced by Angiotensin II and Deoxycorticosterone acetate (DOCA)-high-salt administration, were utilized. CMG was administered at 220 mg/kg daily for four weeks after hypertension onset and blood pressure was measured non-invasively. Whole blood RNA sequencing, metabolomics, real-time PCR, and Western blot analyses of kidney and aorta tissues were additionally performed. Results: CMG significantly lowered systolic, diastolic, and mean blood pressure in both models. RNA sequencing revealed that CMG modulated immunity in the Angiotensin II model and metabolism in the DOCA-HS model. CMG downregulated genes related to oxidative stress and endothelial dysfunction and upregulated endothelial markers such as Vegfa. Metabolomic analysis indicated improved endothelial homeostasis via lysophosphatidylinositol upregulation. Conclusions: CMG emerges as a potent natural antihypertensive therapy, demonstrating beneficial effects on blood pressure and renal endothelial function.
摘要：
背景：高血压造成了巨大的全球健康负担，并与心血管疾病的发病率有关。Chios乳香胶（CMG），源自Pistacialentiscusvar.Chia,显示出作为植物治疗剂的潜力，由于其多方面的有益影响。然而,它的抗高血压作用和血管，循环,和肾脏相关的功能障碍，还没有被彻底调查。在这里，我们旨在探索CMG的降压潜力，关注血管和肾内皮，在体内。方法:两种雄性大鼠高血压模型,血管紧张素II和醋酸脱氧皮质酮（DOCA）-高盐给药诱导，被利用。在高血压发作后，每天以220mg/kg的剂量施用CMG，持续四周，并且无创测量血压。全血RNA测序,代谢组学，实时PCR，此外还进行了肾脏和主动脉组织的Westernblot分析。结果：CMG显著降低收缩压，舒张压,和两个模型的平均血压。RNA测序显示CMG调节血管紧张素II模型中的免疫和DOCA-HS模型中的代谢。CMG下调与氧化应激和内皮功能障碍相关的基因，并上调内皮标志物如Vegfa。代谢组学分析表明通过溶血磷脂酰肌醇上调改善了内皮稳态。结论：CMG作为一种有效的天然抗高血压疗法，显示对血压和肾内皮功能的有益作用。
