PDF(Pubmed)
Abstract：
This study aimed to investigate a synergistic anti-inflammatory effect of a citrus flavonoid nobiletin and docosahexaenoic acid (DHA), one of n-3 long-chain polyunsaturated fatty acids, in combination. Simultaneous treatment with nobiletin and DHA synergistically inhibited nitric oxide production (combination index < 0.9) by mouse macrophage-like RAW 264.7 cells stimulated with lipopolysaccharide (LPS) without cytotoxicity. On the other hand, the inhibitory effect of nobiletin and DHA in combination on proinflammatory cytokine production was not synergistic. Neither nobiletin nor DHA affected the phagocytotic activity of RAW 264.7 cells stimulated with LPS. Immunoblot analysis revealed that the inhibition potency of DHA on the phosphorylation of ERK and p38 and nuclear translocation of NF-κB is markedly enhanced by simultaneously treating with nobiletin, which may lead to the synergistic anti-inflammatory effect. Overall, our findings show the potential of the synergistic anti-inflammatory effect of nobiletin and DHA in combination.
摘要：
本研究旨在研究柑橘类黄酮金胆素和二十二碳六烯酸(DHA)的协同抗炎作用,n-3长链多不饱和脂肪酸之一，在组合。在没有细胞毒性的情况下,用天麻素和DHA同时处理协同抑制用脂多糖(LPS)刺激的小鼠巨噬细胞样RAW264.7细胞产生一氧化氮(组合指数<0.9)。另一方面,川陈皮素和DHA联合对促炎细胞因子产生的抑制作用没有协同作用。景别素和DHA均不影响LPS刺激的RAW264.7细胞的吞噬活性。免疫印迹分析显示，通过同时用山黄精处理，DHA对ERK和p38磷酸化以及NF-κB核易位的抑制作用显着增强。这可能导致协同抗炎作用。总的来说,我们的发现显示了川陈皮素和DHA联合使用具有协同抗炎作用的潜力.
