PDF(Pubmed)
Abstract：
In this study, we investigated the effect of monobutyrin (MB) on the gut microbiota and intestinal health of weaned mice. MB was administered via gavage to 21-day-old weaned mice. Samples of small intestinal and ileal contents were collected on day 1, day 7, and day 21 post-administration. Seven days of MB administration enhanced the mucin layer and morphological structure of the intestine and the integrity of the intestinal brush border. Both MB and sodium butyrate (SB) accelerated tight junction development. Compared to SB, MB modulated intestinal T cells in a distinct manner. MB increased the ratio of Treg cells in the small intestine upon the cessation of weaning. After 21 days of MB administration, enhancement of the villus structure of the ileum was observed. MB increased the proportion of Th17 cells in the ileum. MB facilitated the transition of the small intestinal microbiota toward an adult microbial community structure and enhanced the complexity of the microbial community structure. An increase in Th17 cells enhanced intestinal barrier function. The regulatory effect of MB on Th17 cells may occur through the intestinal microbiota. Therefore, MB can potentially be used to promote intestinal barrier function, especially for weaning animals, with promising application prospects.
摘要：
在这项研究中,我们研究了单丁酸甘油酯（MB）对断奶小鼠肠道菌群和肠道健康的影响。MB通过管饲法给予21日龄断奶小鼠。在施用后第1天、第7天和第21天收集小肠和回肠内容物的样品。施用MB7天增强了肠的粘蛋白层和形态结构以及肠刷状边界的完整性。MB和丁酸钠（SB）都加速了紧密连接的发展。与SB相比，MB以独特的方式调节肠道T细胞。停止断奶后，MB增加了小肠中Treg细胞的比例。MB给药21天后，观察到回肠绒毛结构的增强。MB增加了回肠中Th17细胞的比例。MB促进了小肠微生物区系向成体微生物群落结构的转变，并增强了微生物群落结构的复杂性。Th17细胞的增加增强了肠屏障功能。MB对Th17细胞的调节作用可能通过肠道菌群发生。因此,MB可用于促进肠道屏障功能，尤其是对断奶的动物,具有很好的应用前景。
