PDF(Pubmed)
Abstract：
Excessive vitamin A (VA) negatively impacts bone. Interactions between VA and vitamin D (VD) in bone health are not well-understood. This study used a traditional two-by-two factorial design. Pigs were weaned and randomized to four treatments (n = 13/group): -A-D, -A+D, +A-D, and +A+D for 3 and 5 wk. Serum, liver, kidney, adrenal glands, spleen, and lung were analyzed by ultra-performance LC. Growth was evaluated by weight measured weekly and BMD by DXA. Weights were higher in -A+D (18.1 ± 1.0 kg) and +A+D (18.2 ± 2.3 kg) at 5 wk than in -A-D (15.5 ± 2.1 kg) and +A-D (15.8 ± 1.5 kg). Serum retinol concentrations were 0.25 ± 0.023, 0.22 ± 0.10, 0.77 ± 0.12, and 0.84 ± 0.28 µmol/L; and liver VA concentrations were 0.016 ± 0.015, 0.0065 ± 0.0035, 2.97 ± 0.43, 3.05 ± 0.68 µmol/g in -A-D, -A+D, +A-D, and +A+D, respectively. Serum 25(OH)D3 concentrations were 1.5 ± 1.11, 1.8 ± 0.43, 27.7 ± 8.91, and 23.9 ± 6.67 ng/mL in -A-D, +A-D, -A+D, +A+D, respectively, indicating a deficiency in -D and adequacy in +D. BMD was highest in +D (p < 0.001). VA and the interaction had no effect on BMD. Dietary VD influenced weight gain, BMD, and health despite VA status.
摘要：
过量的维生素A(VA)对骨骼产生负面影响。维生素A和维生素D（VD）在骨骼健康中的相互作用尚不清楚。本研究采用传统的二乘二阶乘设计。猪断奶并随机分为四种处理(n=13/组):-A-D,-A+D,+A-D,3周和5周的+A+D。血清,肝脏,肾,肾上腺,脾,脾和肺进行超性能LC分析。通过每周测量的体重和通过DXA测量的BMD来评估生长。在5周时，-AD（18.1±1.0kg）和AD（18.2±2.3kg）的重量高于-A-D（15.5±2.1kg）和A-D（15.8±1.5kg）。血清视黄醇浓度分别为0.25±0.023、0.22±0.10、0.77±0.12和0.84±0.28µmol/L；在-A-D中，肝脏VA浓度分别为0.016±0.015、0.0065±0.0035、2.97±0.43、3.05±0.68µmol/g，-A+D,+A-D,+A+D,分别。-A-D中的血清25（OH）D3浓度为1.5±1.11、1.8±0.43、27.7±8.91和23.9±6.67ng/mL，+A-D,-A+D,+A+D,分别,表明-D不足，+D充足BMD在+D中最高(p<0.001)。VA和交互作用对BMD没有影响。饮食VD影响体重增加，BMD,和健康，尽管VA状态。
