OBJECTIVE: The objective of this paper was to examine the effect of MI on zebrafish embryos\' locomotor activity, as well as its potential anxiolytic activity in zebrafish larvae.
METHODS: In the first part of the experiment, the embryos were incubated with 5, 10, 20, and 40 mg/mL MI. 1-day post fertilization, embryo mobility was evaluated and burst activity was calculated. In the next part of the study, the behavior of 5-day-old larvae was tested.
RESULTS: Tests on embryo movement showed an increase in burst activity in the MI group at concentrations of 40 mg/mL (p < 0.0001) and a slight decrease in the group at concentrations of 10 mg/mL (p < 0.05). MI in the light/dark challenge had no impact on the thigmotaxis index.
CONCLUSIONS: MI was shown to not affect stress reduction in zebrafish larvae. Further research on the potential of MI and other stereoisomers is needed.
目的:本文的目的是研究MI对斑马鱼胚胎运动活动的影响,以及其在斑马鱼幼虫中的潜在抗焦虑活性。
方法:在实验的第一部分中,用5、10、20和40mg/mLMI孵育胚胎。受精后1天,评估了胚胎的移动性并计算了爆发活性。在研究的下一部分,测试了5日龄幼虫的行为。
结果:胚胎运动试验显示,在浓度为40mg/mL时,MI组的爆发活性增加(p<0.0001),而在浓度为10mg/mL时,MI组的爆发活性略有降低(p<0.05)。亮/暗攻击中的MI对thigmotaxis指数没有影响。
结论:MI不影响斑马鱼幼虫的应激降低。需要进一步研究MI和其他立体异构体的潜力。