PDF(Pubmed)
Abstract：
BACKGROUND: Myo-inositol (MI) is the most abundant inositol found in nature. To date MI supplementation is reported to be effective in the treatment of polycystic ovary syndrome, it is also suggested to alleviate the symptoms of diabetes and neurodegenerative disorders, but to date no statistically significant effects of inositol on depressive and anxiety symptoms were proven. In the study of anxiolytic effects in zebrafish, we often use the thigmotaxis index measuring the ratio of the amount of time the animal spends near the walls compared to the entire arena.
OBJECTIVE: The objective of this paper was to examine the effect of MI on zebrafish embryos\' locomotor activity, as well as its potential anxiolytic activity in zebrafish larvae.
METHODS: In the first part of the experiment, the embryos were incubated with 5, 10, 20, and 40 mg/mL MI. 1-day post fertilization, embryo mobility was evaluated and burst activity was calculated. In the next part of the study, the behavior of 5-day-old larvae was tested.
RESULTS: Tests on embryo movement showed an increase in burst activity in the MI group at concentrations of 40 mg/mL (p < 0.0001) and a slight decrease in the group at concentrations of 10 mg/mL (p < 0.05). MI in the light/dark challenge had no impact on the thigmotaxis index.
CONCLUSIONS: MI was shown to not affect stress reduction in zebrafish larvae. Further research on the potential of MI and other stereoisomers is needed.
OBJECTIVE: The objective of this paper was to examine the effect of MI on zebrafish embryos\' locomotor activity, as well as its potential anxiolytic activity in zebrafish larvae.
METHODS: In the first part of the experiment, the embryos were incubated with 5, 10, 20, and 40 mg/mL MI. 1-day post fertilization, embryo mobility was evaluated and burst activity was calculated. In the next part of the study, the behavior of 5-day-old larvae was tested.
RESULTS: Tests on embryo movement showed an increase in burst activity in the MI group at concentrations of 40 mg/mL (p < 0.0001) and a slight decrease in the group at concentrations of 10 mg/mL (p < 0.05). MI in the light/dark challenge had no impact on the thigmotaxis index.
CONCLUSIONS: MI was shown to not affect stress reduction in zebrafish larvae. Further research on the potential of MI and other stereoisomers is needed.
摘要：
背景：肌醇（MI）是自然界中发现的最丰富的肌醇。迄今为止，据报道补充MI可有效治疗多囊卵巢综合征，还建议减轻糖尿病和神经退行性疾病的症状，但迄今为止，肌醇对抑郁和焦虑症状的影响没有统计学意义。在斑马鱼抗焦虑作用的研究中，我们经常使用thigmotaxis指数来衡量动物在墙壁附近花费的时间与整个竞技场的时间之比。
目的：本文的目的是研究MI对斑马鱼胚胎运动活动的影响，以及其在斑马鱼幼虫中的潜在抗焦虑活性。
方法：在实验的第一部分中，用5、10、20和40mg/mLMI孵育胚胎。受精后1天，评估了胚胎的移动性并计算了爆发活性。在研究的下一部分，测试了5日龄幼虫的行为。
结果：胚胎运动试验显示，在浓度为40mg/mL时，MI组的爆发活性增加（p<0.0001），而在浓度为10mg/mL时，MI组的爆发活性略有降低（p<0.05）。亮/暗攻击中的MI对thigmotaxis指数没有影响。
结论：MI不影响斑马鱼幼虫的应激降低。需要进一步研究MI和其他立体异构体的潜力。
目的：本文的目的是研究MI对斑马鱼胚胎运动活动的影响，以及其在斑马鱼幼虫中的潜在抗焦虑活性。
方法：在实验的第一部分中，用5、10、20和40mg/mLMI孵育胚胎。受精后1天，评估了胚胎的移动性并计算了爆发活性。在研究的下一部分，测试了5日龄幼虫的行为。
结果：胚胎运动试验显示，在浓度为40mg/mL时，MI组的爆发活性增加（p<0.0001），而在浓度为10mg/mL时，MI组的爆发活性略有降低（p<0.05）。亮/暗攻击中的MI对thigmotaxis指数没有影响。
结论：MI不影响斑马鱼幼虫的应激降低。需要进一步研究MI和其他立体异构体的潜力。
