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Abstract：
Stem color is an important agronomic trait of wax gourds. However, its regulatory genes have not been identified. In this study, 105 inbred lines constructed from two parents (GX-71 and MY-1) were sequenced and quantitative trait loci sequencing was used to mine the genes that regulate stem color in wax gourds. The results identified two quantitative trait loci related to stem color, qSC5 and qSC12, located on Chr05 (11,134,567-16,459,268) and Chr12 (74,618,168-75,712,335), respectively. The qSC5 had a phenotypic variation rate of 36.9% and a maximum limit of detection of 16.9. And the qSC12 had a phenotypic variation rate of 20.9%, and a maximum limit of detection of 11.2. Bch05G003950 (named BchAPRR2) and Bch12G020400 were identified as candidate genes involved in stem color regulation in wax gourds. The chlorophyll content and expression of BchAPRR2 and Bch12G020400 were significantly higher in green-stemmed wax gourds than in white-stemmed ones. Therefore, BchAPRR2 and Bch12G020400 were considered the main and secondary regulatory genes for wax gourd stem color, respectively. Finally, InDel markers closely linked to BchAPRR2 were developed to validate the prediction of wax gourd stem color traits in 55 germplasm lines, with an accuracy of 81.8%. These findings lay the foundation for exploring the genetic regulation of wax gourd stem color and future research on wax gourd breeding.
摘要：
茎色是冬瓜的重要农艺性状。然而,其调控基因尚未被鉴定。在这项研究中,对由两个亲本（GX-71和MY-1）构建的105个自交系进行了测序，并使用数量性状基因座测序来挖掘调节冬瓜茎色的基因。结果确定了两个与茎颜色相关的数量性状位点，qSC5和qSC12，位于Chr05（11,134,567-16,459,268）和Chr12（74,618,168-75,712,335），分别。qSC5的表型变异率为36.9%,最大检测限为16.9。qSC12的表型变异率为20.9%,最大检出限为11.2。Bch05G003950（命名为BchAPRR2）和Bch12G020400被鉴定为参与冬瓜茎色调节的候选基因。绿茎冬瓜的叶绿素含量和BchAPRR2和Bch12G020400的表达明显高于白茎冬瓜。因此,BchAPRR2和Bch12G020400被认为是冬瓜茎色的主要和次要调控基因，分别。最后,开发了与BchAPRR2密切相关的InDel标记，以验证55个种质系冬瓜茎色性状的预测，准确率为81.8%。这些发现为探索冬瓜茎色的遗传调控和今后冬瓜育种研究奠定了基础。
