PDF(Pubmed)
Abstract：
Prolonged exposure to environmental oxidative stress can result in visible signs of skin aging such as wrinkles, hyperpigmentation, and thinning of the skin. Oryza sativa variety Sang 5 CMU, an inbred rice cultivar from northern Thailand, contains phenolic and flavonoid compounds in its bran and husk portions that are known for their natural antioxidant properties. In this study, we evaluated the cosmetic properties of crude extracts from rice bran and husk of Sang 5 CMU, focusing on antioxidant, anti-inflammatory, anti-melanogenesis, and collagen-regulating properties. Our findings suggest that both extracts possess antioxidant potential against DPPH, ABTS radicals, and metal ions. Additionally, they could downregulate TBARS levels from 125% to 100% of the control, approximately, while increasing the expression of genes related to the NRF2-mediated antioxidant pathway, such as NRF2 and HO-1, in H2O2-induced human fibroblast cells. Notably, rice bran and husk extracts could increase mRNA levels of HO-1 more greatly than the standard L-ascorbic acid, by about 1.29 and 1.07 times, respectively. Furthermore, the crude extracts exhibited anti-inflammatory activity by suppressing nitric oxide production in both mouse macrophage and human fibroblast cells. Specifically, the bran and husk extracts inhibited the gene expression of the inflammatory cytokine IL-6 in LPS-induced inflammation in fibroblasts. Moreover, both extracts demonstrated potential for inhibiting melanin production and intracellular tyrosinase activity in human melanoma cells by decreasing the expression of the transcription factor MITF and the pigmentary genes TYR, TRP-1, and DCT. They also exhibit collagen-stimulating effects by reducing MMP-2 expression in H2O2-induced fibroblasts from 135% to 80% of the control, approximately, and increasing the gene associated with type I collagen production, COL1A1. Overall, the rice bran and husk extracts of Sang 5 CMU showed promise as effective natural ingredients for cosmetic applications.
摘要：
长时间暴露于环境氧化应激会导致皮肤老化的明显迹象，如皱纹，色素沉着过度,皮肤变薄。水稻品种Sang5CMU，来自泰国北部的近交水稻品种，在麸皮和外皮部分含有酚类和类黄酮化合物，以其天然抗氧化特性而闻名。在这项研究中,我们评估了Sang5CMU的米糠和外壳粗提物的化妆品特性，专注于抗氧化剂，抗炎,抗黑色素生成，和胶原蛋白调节特性。我们的研究结果表明，两种提取物都具有抗DPPH的抗氧化潜力，ABTS自由基，和金属离子。此外,他们可以将TBARS水平从控制的125%下调到100%，大约,同时增加NRF2介导的抗氧化途径相关基因的表达，例如NRF2和HO-1，在H2O2诱导的人成纤维细胞中。值得注意的是,米糠和稻壳提取物可以比标准L-抗坏血酸更大地增加HO-1的mRNA水平，大约1.29和1.07倍，分别。此外，粗提物通过抑制小鼠巨噬细胞和人成纤维细胞中一氧化氮的产生而表现出抗炎活性。具体来说,麸皮和皮提取物抑制了LPS诱导的成纤维细胞炎症中炎症细胞因子IL-6的基因表达。此外，两种提取物都显示出通过降低转录因子MITF和色素基因TYR的表达来抑制人黑色素瘤细胞中黑色素的产生和细胞内酪氨酸酶活性的潜力，TRP-1和DCT。它们还通过将H2O2诱导的成纤维细胞中的MMP-2表达从对照的135％降低到80％而表现出胶原蛋白刺激作用。大约,增加与I型胶原蛋白产生相关的基因，COL1A1.总的来说,Sang5CMU的米糠和果壳提取物有望成为化妆品应用的有效天然成分。
