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Abstract：
Bassia indica (Wight) A.J. Scott is an Indian origin plant with documented medicinal and nutritional value, but has not been fully characterized yet. The present study was designed to establish pharmacognostic standards for the proper identification of the B. indica plant and its chemical characterization. The plant was standardized with World Health Organization (WHO) standardization tools and chemically characterized by Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectroscopy (GC-MS) analysis. Antibacterial potential was assessed by the zone of inhibition and minimum inhibitory concentration (MIC), and molecular docking studies were also performed. Pharmacognostic evaluation established the macroscopic and microscopic parameters for the identification of whole plant and its powder. Physicochemical parameters were also set forth while quantitative phytochemical analysis showed that the ethyl acetate fraction had the highest quantity of phenols, flavonoids, and tannins. FTIR analysis showed several functional groups such as phenols, alkanes, and alcohols while 55 phytochemicals were identified in the GC-MS analysis of the crude fraction. The crude extract and other fractions showed marked antibacterial activity, while the ethyl acetate fraction showed the least MIC (1.95-31.25 mg/mL). Phytochemicals identified in the GC-MS showed good molecular docking interactions against the DNA gyrase subunit B of bacteria with binding energies ranging from -4.2 to -9.4 kcal/mol. The current study describes the pharmacognostic characterization and phytochemical profiling of B. indica and provides scientific evidence to support its use in infections.
摘要：
印度巴西亚（怀特）A.J.斯科特是一种印度起源的植物，有文献记载的药用和营养价值，但还没有完全描述。本研究旨在建立生药学标准，以正确鉴定B.in属植物及其化学表征。该工厂用世界卫生组织（WHO）标准化工具进行了标准化，并通过傅立叶变换红外光谱（FTIR）和气相色谱-质谱（GC-MS）分析进行了化学表征。通过抑制区和最小抑制浓度（MIC）评估抗菌潜力，和分子对接研究也进行了。生药学评估建立了用于鉴定整个植物及其粉末的宏观和微观参数。物理化学参数也进行了阐述，而定量植物化学分析表明，乙酸乙酯部分的酚含量最高，黄酮类化合物，还有单宁.FTIR分析显示几个官能团，如酚类，烷烃,和醇，而在粗馏分的GC-MS分析中鉴定出55种植物化学物质。粗提物和其他部分显示出明显的抗菌活性，而乙酸乙酯部分显示最小的MIC(1.95-31.25mg/mL)。在GC-MS中鉴定的植物化学物质显示出与细菌的DNA促旋酶亚基B的良好分子对接相互作用，结合能范围为-4.2至-9.4kcal/mol。目前的研究描述了B.indea的生药学特征和植物化学分析，并提供了科学证据来支持其在感染中的使用。
