PDF(Pubmed)
Abstract：
The evolved resistance of Bromus japonicus Houtt. to ALS-inhibiting herbicides is well established. Previous studies have primarily focused on target-site resistance; however, non-target-site resistance has not been well characterized. This investigation demonstrated that ALS gene sequencing did not detect any previously known resistance mutations in a mesosulfuron-methyl-resistant (MR) population, and notably, treatment with the P450 monooxygenase (P450) inhibitor malathion markedly heightened susceptibility to mesosulfuron-methyl. Utilizing UPLC-MS/MS analysis confirmed elevated mesosulfuron-methyl metabolism in MR plants. The integration of Isoform Sequencing (Iso-Seq) and RNA Sequencing (RNA-Seq) facilitated the identification of candidate genes associated with non-target sites in a subpopulation with two generations of herbicide selection. Through qRT-PCR analysis, 21 differentially expressed genes were characterized, and among these, 10 genes (comprising three P450s, two glutathione S-transferases, one glycosyltransferase, two ATP-binding cassette transporters, one oxidase, and one hydrolase) exhibited constitutive upregulation in resistant plants. Our findings substantiated that increased herbicide metabolism is a driving force behind mesosulfuron-methyl resistance in this B. japonicus population.
摘要：
日本BromusHoutt的进化抗性。抑制ALS的除草剂是公认的。以前的研究主要集中在目标位点抗性;然而，非目标位点抗性尚未得到很好的表征。这项研究表明，ALS基因测序未检测到甲磺隆耐药（MR）人群中任何先前已知的耐药突变，尤其是,用P450单加氧酶（P450）抑制剂马拉硫磷治疗可显着提高对甲磺隆的敏感性。利用UPLC-MS/MS分析证实了MR植物中甲磺隆-甲基代谢升高。同种型测序(Iso-Seq)和RNA测序(RNA-Seq)的整合促进了具有两代除草剂选择的亚群中与非靶位点相关的候选基因的鉴定。通过qRT-PCR分析,对21个差异表达基因进行了表征，其中，10个基因（包括三个P450，两种谷胱甘肽S-转移酶，一种糖基转移酶，两个ATP结合盒转运蛋白，一种氧化酶，和一种水解酶）在抗性植物中表现出组成性上调。我们的发现证实，除草剂代谢的增加是该日本芽孢杆菌种群中甲磺隆抗性的驱动力。
