PDF(Pubmed)
Abstract：
In this study, we collected seven prevalent Taiwanese Desmodium plants, including three species with synonymous characteristics, in order to assess their antioxidant phytoconstituents and radical scavenging capacities. Additionally, we compared their inhibitory activities on monoamine oxidase (MAO) and 6-hydroxydopamine (6-OHDA) auto-oxidation. Subsequently, we evaluated the neuroprotective potential of D. pulchellum on 6-OHDA-induced nerve damage in SH-SY5Y cells and delved into the underlying neuroprotective mechanisms. Among the seven Desmodium species, D. pulchellum exhibited the most robust ABTS radical scavenging capacity and relative reducing power; correspondingly, it had the highest total phenolic and phenylpropanoid contents. Meanwhile, D. motorium showcased the best hydrogen peroxide scavenging capacity and, notably, D. sequax demonstrated remarkable prowess in DPPH radical and superoxide scavenging capacity, along with selective inhibitory activity against MAO-B. Of the aforementioned species, D. pulchellum emerged as the frontrunner in inhibiting 6-OHDA auto-oxidation and conferring neuroprotection against 6-OHDA-induced neuronal damage in the SH-SY5Y cells. Furthermore, D. pulchellum effectively mitigated the increase in intracellular ROS and MDA levels through restoring the activities of the intracellular antioxidant defense system. Therefore, we suggest that D. pulchellum possesses neuroprotective effects against 6-OHDA-induced neurotoxicity due to the radical scavenging capacity of its antioxidant phytoconstituents and its ability to restore intracellular antioxidant activities.
摘要：
在这项研究中,我们收集了7种流行的台湾Desmodium植物，包括三个具有同义特征的物种，以评估其抗氧化植物成分和自由基清除能力。此外,我们比较了它们对单胺氧化酶（MAO）和6-羟基多巴胺（6-OHDA）自氧化的抑制活性。随后，我们评估了D.pulchel对6-OHDA诱导的SH-SY5Y细胞神经损伤的神经保护潜力，并探讨了潜在的神经保护机制。在七个Desmodium物种中，D.Pulchellum表现出最强大的ABTS自由基清除能力和相对还原能力；相应地，它具有最高的总酚类和苯丙素含量。同时,D.motorium展示了最佳的过氧化氢清除能力，特别是,D.sequax在DPPH自由基和超氧化物清除能力方面表现出非凡的能力，以及对MAO-B的选择性抑制活性。在上述物种中，D.髓细胞在抑制6-OHDA自氧化并赋予神经保护以抵抗SH-SY5Y细胞中6-OHDA诱导的神经元损伤方面成为前沿分子。此外，D.通过恢复细胞内抗氧化防御系统的活性，有效地减轻了细胞内ROS和MDA水平的增加。因此,我们建议D.pulchellum具有针对6-OHDA诱导的神经毒性的神经保护作用，这是由于其抗氧化植物成分的自由基清除能力及其恢复细胞内抗氧化活性的能力。
