PDF(Pubmed)
Abstract：
Gluconacetobacter diazotrophicus is a diazotrophic endophytic bacterium that promotes the growth and development of several plant species. However, the molecular mechanisms activated during plant response to this bacterium remain unclear. Here, we used the RNA-seq approach to understand better the effect of G. diazotrophicus PAL5 on the transcriptome of shoot and root tissues of Arabidopsis thaliana. G. diazotrophicus colonized A. thaliana roots and promoted growth, increasing leaf area and biomass. The transcriptomic analysis revealed several differentially expressed genes (DEGs) between inoculated and non-inoculated plants in the shoot and root tissues. A higher number of DEGs were up-regulated in roots compared to shoots. Genes up-regulated in both shoot and root tissues were associated with nitrogen metabolism, production of glucosinolates and flavonoids, receptor kinases, and transcription factors. In contrast, the main groups of down-regulated genes were associated with pathogenesis-related proteins and heat-shock proteins in both shoot and root tissues. Genes encoding enzymes involved in cell wall biogenesis and modification were down-regulated in shoots and up-regulated in roots. In contrast, genes associated with ROS detoxification were up-regulated in shoots and down-regulated in roots. These results highlight the fine-tuning of the transcriptional regulation of A. thaliana in response to colonization by G. diazotrophicus PAL5.
摘要：
重氮葡糖醋杆菌是一种重氮营养内生细菌，可促进几种植物的生长和发育。然而,植物对这种细菌反应过程中激活的分子机制仍不清楚。这里,我们使用RNA-seq方法来更好地了解重氮G.DAL5对拟南芥芽和根组织转录组的影响。G.重氮营养菌定植了拟南芥的根并促进了生长，增加叶面积和生物量。转录组学分析揭示了芽和根组织中接种和未接种植物之间的几个差异表达基因（DEGs）。与芽相比，根中更多的DEG上调。在芽和根组织中上调的基因与氮代谢有关，生产芥子油苷和类黄酮，受体激酶，和转录因子。相比之下,下调的主要基因与芽和根组织中的发病相关蛋白和热休克蛋白相关。编码参与细胞壁生物发生和修饰的酶的基因在芽中下调，在根中上调。相比之下,与ROS解毒相关的基因在芽中上调，在根中下调。这些结果强调了响应于重氮营养G.PAL5定殖的拟南芥的转录调节的微调。
