PDF(Pubmed)
Abstract：
Background/Objectives: Periodontitis is caused by bacterial plaque. The oral microflora may interact with the intestinal microflora and play a role in the development of periodontitis. The periodontal inflamed surface area (PISA) has been shown to be a useful indicator of periodontal disease related to systemic diseases; however, few studies have shown an association between PISA and the bacterial flora. This study aimed to determine the association between PISA and oral and intestinal bacteria. Methods: Participants were recruited between 2018 and 2021 at the Medical and Dental Collaboration Center of Kanagawa Dental University Hospital. A periodontal clinical examination was performed, and the PISA was calculated. Salivary tests were conducted, and leukocyte scores in the saliva were calculated. Moreover, 16S rRNA amplicon sequencing was performed using saliva and stool samples to analyze oral and intestinal bacteria, respectively. Results: Higher PISA levels resulted in an increased presence of Bacteroides and a decreased presence of Proteobacteria and Actinobacteria in the saliva. An increase in Bacteroides was detected in the saliva of patients with high leukocyte scores. No correlation was observed between PISA and intestinal bacteria. Conclusions: Bacteroides was highly abundant in the saliva of patients with worsened periodontal conditions, as indicated by PISA. No association was found between PISA and intestinal bacteria.
摘要：
背景/目的：牙周炎是由细菌菌斑引起的。口腔菌群可能与肠道菌群相互作用，在牙周炎的发展中起作用。牙周发炎表面积（PISA）已被证明是与全身性疾病相关的牙周病的有用指标；但是，很少有研究表明PISA与细菌菌群之间存在关联。本研究旨在确定PISA与口腔和肠道细菌之间的关联。方法：2018年至2021年在神奈川牙科大学附属医院医疗与牙科合作中心招募参与者。进行了牙周临床检查，并计算了PISA。进行了唾液测试，计算唾液中的白细胞评分。此外，使用唾液和粪便样本进行16SrRNA扩增子测序，以分析口腔和肠道细菌，分别。结果：较高的PISA水平导致唾液中拟杆菌的存在增加，以及变形杆菌和放线菌的存在减少。在具有高白细胞评分的患者的唾液中检测到拟杆菌的增加。PISA与肠道细菌之间没有相关性。结论：牙周恶化患者的唾液中类杆菌含量很高，如PISA所示。在PISA和肠道细菌之间没有发现关联。
