PDF(Pubmed)
Abstract：
In this study, a liquid chromatographic method was developed for the fast determination of lincomycin, polymyxin and vancomycin in a preservation solution for transplants. A Kinetex EVO C18 (150 × 4.6 mm, 2.6 µm) column was utilized at 45 °C. Gradient elution was applied using a mixture of mobile phases A and B, both including 30 mM phosphate buffer at pH 2.0 and acetonitrile, at a ratio of 95:5 (v/v) for A and 50:50 (v/v) for B. A flow rate of 1.0 mL/min, an injection volume of 20 µL and UV detection at 210 nm were used. A degradation study treating the three antibiotics with 0.5 M hydrochloric acid, 0.5 M sodium hydroxide and 3% H2O2 indicated that the developed method was selective toward lincomycin, polymyxin, vancomycin and their degradation products. Other ingredients of the preservation solution, like those from the cell culture medium, did not interfere. The method was validated with good sensitivity, linearity, precision and accuracy. Furthermore, lincomycin, polymyxin and vancomycin were found to be stable in this preservation solution for 4 weeks when stored at -20 °C.
摘要：
在这项研究中,建立了一种快速测定林可霉素的液相色谱法，多粘菌素和万古霉素在移植保存溶液中。KinetexEVOC18(150×4.6mm,2.6µm)色谱柱在45°C下使用使用流动相A和B的混合物进行梯度洗脱，均包括pH2.0的30mM磷酸盐缓冲液和乙腈，A的比例为95：5（v/v），B的比例为50：50（v/v）。流速为1.0mL/min，使用20μL的注射体积和210nm的UV检测。用0.5M盐酸处理三种抗生素的降解研究，0.5M氢氧化钠和3%H2O2表明所开发的方法对林可霉素具有选择性，多粘菌素,万古霉素及其降解产物。保存液的其他成分，就像那些来自细胞培养基的，没有干涉。方法经过验证,具有良好的灵敏度,线性度精度和准确性。此外，林可霉素,发现多粘菌素和万古霉素在该保存溶液中在-20°C下储存4周时是稳定的。
