PDF(Pubmed)
Abstract：
This study delves into the chemical and genetic determinants of petal color and fragrance in Rosa canina L., a wild rose species prized for its pharmacological and cosmetic uses. Comparative analysis of white and dark pink R. canina flowers revealed that the former harbors significantly higher levels of total phenolics (TPC) and flavonoids (TFC), while the latter is distinguished by elevated total anthocyanins (TAC). Essential oils in the petals were predominantly composed of aliphatic hydrocarbons, with phenolic content chiefly constituted by flavonols and anthocyanins. Notably, gene expression analysis showed an upregulation in most genes associated with petal color and scent biosynthesis in white buds compared to dark pink open flowers. However, anthocyanin synthase (ANS) and its regulatory gene RhMYB1 exhibited comparable expression levels across both flower hues. LC-MS profiling identified Rutin, kaempferol, quercetin, and their derivatives as key flavonoid constituents, alongside cyanidin and delphinidin as the primary anthocyanin compounds. The findings suggest a potential feedback inhibition of anthocyanin biosynthesis in white flowers. These insights pave the way for the targeted enhancement of R. canina floral traits through metabolic and genetic engineering strategies.
摘要：
这项研究探讨了RosacaninaL.花瓣颜色和香气的化学和遗传决定因素，一种因其药理和化妆品用途而备受赞誉的野玫瑰。对白色和深粉红色R.canina花的比较分析表明，前者的总酚（TPC）和类黄酮（TFC）含量显着升高，而后者的特点是总花色苷（TAC）升高。花瓣中的精油主要由脂肪烃组成，酚类物质主要由黄酮醇和花色苷组成。值得注意的是,基因表达分析显示，与深粉红色开放花相比，白色芽中与花瓣颜色和气味生物合成相关的大多数基因上调。然而,花青素合成酶（ANS）及其调节基因RhMYB1在两种花色中的表达水平相当。LC-MS分析鉴定了芦丁，山奈酚,槲皮素,以及它们的衍生物作为关键的类黄酮成分，与花色素苷和飞燕草苷一起作为主要的花色素苷化合物。研究结果表明，白花中花青素生物合成的潜在反馈抑制作用。这些见解为通过代谢和基因工程策略有针对性地增强R.canina花特性铺平了道路。
