PDF(Pubmed)
Abstract：
BACKGROUND: As one of the four most valuable animal medicines, Fel Ursi, named Xiong Dan (XD) in China, has the effect of clearing heat, calming the liver, and brightening the eyes. However, due to the special source of XD and its high price, other animals\' bile is often sold as XD or mixed with XD on the market, seriously affecting its clinical efficacy and consumers\' rights and interests. In order to realize identification and adulteration analysis of XD, UHPLC-QTOF-MSE and multivariate statistical analysis were used to explore the differences in XD and six other animals\' bile.
METHODS: XD, pig gall (Zhu Dan, ZD), cow gall (Niu Dan, ND), rabbit gallbladder (Tu Dan, TD), duck gall (Yan Dan, YD), sheep gall (Yang Dan, YND), and chicken gall (Ji Dan, JD) were analyzed by UHPLC-QTOF-MSE, and the MS data, combined with multivariate analysis methods, were used to distinguish between them. Meanwhile, the potential chemical composition markers that contribute to their differences were further explored.
RESULTS: The results showed that XD and six other animals\' bile can be distinguished from each other obviously, with 27 ions with VIP > 1.0. We preliminarily identified 10 different bile acid-like components in XD and the other animals\' bile with significant differences (p < 0.01) and VIP > 1.0, such as tauroursodeoxycholic acid, Glycohyodeoxycholic acid, and Glycodeoxycholic acid.
CONCLUSIONS: The developed method was efficient and rapid in accurately distinguishing between XD and six other animals\' bile. Based on the obtained chemical composition markers, it is beneficial to strengthen quality control for bile medicines.
METHODS: XD, pig gall (Zhu Dan, ZD), cow gall (Niu Dan, ND), rabbit gallbladder (Tu Dan, TD), duck gall (Yan Dan, YD), sheep gall (Yang Dan, YND), and chicken gall (Ji Dan, JD) were analyzed by UHPLC-QTOF-MSE, and the MS data, combined with multivariate analysis methods, were used to distinguish between them. Meanwhile, the potential chemical composition markers that contribute to their differences were further explored.
RESULTS: The results showed that XD and six other animals\' bile can be distinguished from each other obviously, with 27 ions with VIP > 1.0. We preliminarily identified 10 different bile acid-like components in XD and the other animals\' bile with significant differences (p < 0.01) and VIP > 1.0, such as tauroursodeoxycholic acid, Glycohyodeoxycholic acid, and Glycodeoxycholic acid.
CONCLUSIONS: The developed method was efficient and rapid in accurately distinguishing between XD and six other animals\' bile. Based on the obtained chemical composition markers, it is beneficial to strengthen quality control for bile medicines.
摘要：
背景：作为四种最有价值的动物药物之一，FelUrsi,在中国叫熊丹（XD），具有清除热量的作用，平静肝脏，和明亮的眼睛。然而,由于XD的特殊来源和高昂的价格，其他动物胆汁通常以XD或与XD混合在市场上出售，严重影响其临床疗效和消费者权益。为了实现对XD的鉴别和掺假分析,UHPLC-QTOF-MSE和多变量统计分析用于探索XD和其他6只动物胆汁的差异。
方法：XD，猪胆(朱丹，ZD),牛胆(牛丹，ND),兔胆囊(涂丹,TD),鸭胆(颜丹,YD),羊胆(杨丹，YND),和鸡胆(吉丹，JD)通过UHPLC-QTOF-MSE分析，和MS数据，结合多变量分析方法，被用来区分它们。同时,进一步探索了导致它们差异的潜在化学成分标记。
结果：结果表明，XD和其他六只动物的胆汁可以明显区分开来，与27个离子与VIP>1.0。我们初步鉴定了XD和其他动物胆汁中10种不同的胆汁酸样成分，具有显着差异（p<0.01）和VIP>1.0，例如牛磺熊去氧胆酸，糖脱氧胆酸，和糖脱氧胆酸。
结论：所开发的方法可以有效，快速地准确区分XD和其他六种动物的胆汁。根据获得的化学成分标记，有利于加强胆汁类药品的质量控制。
方法：XD，猪胆(朱丹，ZD),牛胆(牛丹，ND),兔胆囊(涂丹,TD),鸭胆(颜丹,YD),羊胆(杨丹，YND),和鸡胆(吉丹，JD)通过UHPLC-QTOF-MSE分析，和MS数据，结合多变量分析方法，被用来区分它们。同时,进一步探索了导致它们差异的潜在化学成分标记。
结果：结果表明，XD和其他六只动物的胆汁可以明显区分开来，与27个离子与VIP>1.0。我们初步鉴定了XD和其他动物胆汁中10种不同的胆汁酸样成分，具有显着差异（p<0.01）和VIP>1.0，例如牛磺熊去氧胆酸，糖脱氧胆酸，和糖脱氧胆酸。
结论：所开发的方法可以有效，快速地准确区分XD和其他六种动物的胆汁。根据获得的化学成分标记，有利于加强胆汁类药品的质量控制。
