PDF(Pubmed)
Abstract：
Coatings with xenogenic materials, made of detonation nanodiamonds, provide additional strength and increase elasticity. A functionally developed surface of nanodiamonds makes it possible to apply antibiotics. Previous experiments show the stability of such coatings; however, studies on stability in the bloodstream and calcification of the material in natural conditions have yet to be conducted. Tritium-labeled nanodiamonds (negative and positive) were obtained by the tritium activation method and used to develop coatings for a pork aorta to analyze their stability in a pig\'s bloodstream using a radiotracer technique. A chitosan layer was applied from a solution of carbonic acid under high-pressure conditions to prevent calcification. The obtained materials were used to prepare a porcine conduit, which was surgically stitched inside the pig\'s aorta for four months. The aorta samples, including nanodiamond-coated and control samples, were analyzed for nanodiamond content and calcium, using the radiotracer and ICP-AES methods. A histological analysis of the materials was also performed. The obtained coatings illustrate a high in vivo stability and low levels of calcification for all types of nanodiamonds. Even though we did not use additional antibiotics in this case, the development of infection was not observed for negatively charged nanodiamonds, opening up prospects for their use in developing coatings.
摘要：
异种材料涂层，由爆炸纳米金刚石制成，提供额外的强度和增加弹性。纳米金刚石的功能发育的表面使得应用抗生素成为可能。以前的实验表明这种涂层的稳定性;然而，关于自然条件下血液稳定性和材料钙化的研究尚未进行。通过tri活化方法获得tri标记的纳米金刚石（阴性和阳性），并用于开发猪肉主动脉的涂层，以使用放射性示踪剂技术分析其在猪血流中的稳定性。在高压条件下从碳酸溶液中施加壳聚糖层以防止钙化。获得的材料用于制备猪导管，通过手术在猪的主动脉内缝合了四个月。主动脉样本,包括纳米金刚石涂层和对照样品，分析了纳米金刚石含量和钙，使用放射性示踪剂和ICP-AES方法。还进行了材料的组织学分析。对于所有类型的纳米金刚石,所获得的涂层显示出高的体内稳定性和低水平的钙化。即使我们在这种情况下没有使用额外的抗生素，对于带负电荷的纳米金刚石，没有观察到感染的发展，为它们在开发涂料中的应用开辟了前景。
