PDF(Pubmed)
Abstract：
Hypochlorite (ClO-) and viscosity both affect the physiological state of mitochondria, and their abnormal levels are closely related to many common diseases. Therefore, it is vitally important to develop mitochondria-targeting fluorescent probes for the dual sensing of ClO- and viscosity. Herein, we have explored a new fluorescent probe, XTAP-Bn, which responds sensitively to ClO- and viscosity with off-on fluorescence changes at 558 and 765 nm, respectively. Because the emission wavelength gap is more than 200 nm, XTAP-Bn can effectively eliminate the signal crosstalk during the simultaneous detection of ClO- and viscosity. In addition, XTAP-Bn has several advantages, including high selectivity, rapid response, good water solubility, low cytotoxicity, and excellent mitochondrial-targeting ability. More importantly, probe XTAP-Bn is successfully employed to monitor the dynamic change in ClO- and viscosity levels in the mitochondria of living cells and zebrafish. This study not only provides a reliable tool for identifying mitochondrial dysfunction but also offers a potential approach for the early diagnosis of mitochondrial-related diseases.
摘要：
次氯酸盐(ClO-)和粘度都影响线粒体的生理状态,它们的异常水平与许多常见疾病密切相关。因此,开发线粒体靶向荧光探针对ClO-和粘度的双重传感至关重要。在这里，我们探索了一种新的荧光探针，XTAP-Bn,它对ClO-和粘度敏感地响应，在558和765nm处发生关断荧光变化，分别。因为发射波长间隙大于200nm,XTAP-Bn可以有效消除ClO-和粘度同时检测过程中的信号串扰。此外,XTAP-Bn有几个优点，包括高选择性，快速反应,良好的水溶性，低细胞毒性，和出色的线粒体靶向能力。更重要的是,XTAP-Bn探针已成功用于监测活细胞和斑马鱼线粒体中ClO-和粘度水平的动态变化。这项研究不仅为识别线粒体功能障碍提供了可靠的工具，而且为线粒体相关疾病的早期诊断提供了潜在的方法。
