PDF(Pubmed)
Abstract：
BACKGROUND: Lincomycin (LIN) is extensively used for treating diseases in livestock and promoting growth in food animal farming, and it is frequently found in both the environment and in food products. Currently, most of the methods for detecting lincomycin either lack sensitivity and precision or require the use of costly equipment such as mass spectrometers.
RESULTS: In this study, we developed a reliable high-performance liquid chromatography-ultraviolet detection (HPLC-UVD) method and used it to detect LIN residue in 11 types of matrices (pig liver and muscle; chicken kidney and liver; cow fat, liver and milk; goat muscle, liver and milk; and eggs) for the first time. The tissue homogenates and liquid samples were extracted via liquid-liquid extraction, and subsequently purified and enriched via sorbent and solid phase extraction (SPE). After nitrogen drying, the products were derivatized with p-toluene sulfonyl isocyanic acid (PTSI) (100 µL) for 30 min at room temperature. Finally, the derivatized products were analyzed by HPLC at 227 nm. Under the optimized conditions, the method displayed impressive performance and demonstrated its reliability and practicability, with a limit of detection (LOD) and quantification (LOQ) of LIN in each matrix of 25-40 μg/kg and 40-60 μg/kg, respectively. The recovery ranged from 71.11% to 98.30%.
CONCLUSIONS: The results showed that this method had great selectivity, high sensitivity, satisfactory recovery and cost-effectiveness-fulfilling the criteria in drug residue and actual detection requirements-and proved to have broad applicability in the field of detecting LIN in animal-derived foods.
RESULTS: In this study, we developed a reliable high-performance liquid chromatography-ultraviolet detection (HPLC-UVD) method and used it to detect LIN residue in 11 types of matrices (pig liver and muscle; chicken kidney and liver; cow fat, liver and milk; goat muscle, liver and milk; and eggs) for the first time. The tissue homogenates and liquid samples were extracted via liquid-liquid extraction, and subsequently purified and enriched via sorbent and solid phase extraction (SPE). After nitrogen drying, the products were derivatized with p-toluene sulfonyl isocyanic acid (PTSI) (100 µL) for 30 min at room temperature. Finally, the derivatized products were analyzed by HPLC at 227 nm. Under the optimized conditions, the method displayed impressive performance and demonstrated its reliability and practicability, with a limit of detection (LOD) and quantification (LOQ) of LIN in each matrix of 25-40 μg/kg and 40-60 μg/kg, respectively. The recovery ranged from 71.11% to 98.30%.
CONCLUSIONS: The results showed that this method had great selectivity, high sensitivity, satisfactory recovery and cost-effectiveness-fulfilling the criteria in drug residue and actual detection requirements-and proved to have broad applicability in the field of detecting LIN in animal-derived foods.
摘要：
背景：林可霉素（LIN）广泛用于治疗牲畜疾病和促进食用动物养殖的生长，它经常出现在环境和食品中。目前,大多数检测林可霉素的方法要么缺乏灵敏度和精度，要么需要使用昂贵的设备，如质谱仪。
结果：在这项研究中，我们开发了一种可靠的高效液相色谱-紫外检测（HPLC-UVD）方法，并将其用于检测11种基质中的LIN残留（猪肝和肌肉；鸡肾和肝脏；牛脂肪，肝脏和牛奶；山羊肌肉，肝脏和牛奶；和鸡蛋）第一次。通过液-液萃取提取组织匀浆和液体样品，随后通过吸附剂和固相萃取（SPE）纯化和富集。氮气干燥后，产物在室温下用对甲苯磺酰基异氰酸(PTSI)(100微升)衍生30分钟。最后,通过HPLC在227nm处分析衍生化产物。在优化条件下,该方法表现出了令人印象深刻的性能，证明了其可靠性和实用性，每个基质中LIN的检测限（LOD）和定量限（LOQ）为25-40μg/kg和40-60μg/kg，分别。回收率为71.11%~98.30%。
结论：结果表明，该方法具有很好的选择性，高灵敏度,令人满意的回收率和成本效益-满足药物残留标准和实际检测要求-并被证明在检测动物源性食品中的LIN领域具有广泛的适用性。
结果：在这项研究中，我们开发了一种可靠的高效液相色谱-紫外检测（HPLC-UVD）方法，并将其用于检测11种基质中的LIN残留（猪肝和肌肉；鸡肾和肝脏；牛脂肪，肝脏和牛奶；山羊肌肉，肝脏和牛奶；和鸡蛋）第一次。通过液-液萃取提取组织匀浆和液体样品，随后通过吸附剂和固相萃取（SPE）纯化和富集。氮气干燥后，产物在室温下用对甲苯磺酰基异氰酸(PTSI)(100微升)衍生30分钟。最后,通过HPLC在227nm处分析衍生化产物。在优化条件下,该方法表现出了令人印象深刻的性能，证明了其可靠性和实用性，每个基质中LIN的检测限（LOD）和定量限（LOQ）为25-40μg/kg和40-60μg/kg，分别。回收率为71.11%~98.30%。
结论：结果表明，该方法具有很好的选择性，高灵敏度,令人满意的回收率和成本效益-满足药物残留标准和实际检测要求-并被证明在检测动物源性食品中的LIN领域具有广泛的适用性。
