PDF(Pubmed)
Abstract：
In our search for a biocompatible composite hemostatic dressing, we focused on the design of a novel biomaterial composed of two natural biological components, collagen and sodium alginate (SA), cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) and oxidized sodium alginate (OSA). We conducted a series of tests to evaluate the physicochemical properties, acute systemic toxicity, skin irritation, intradermal reaction, sensitization, cytotoxicity, and in vivo femoral artery hemorrhage model. The results demonstrated the excellent biocompatibility of the collagen/sodium alginate (C/SA)-based dressings before and after crosslinking. Specifically, the femoral artery hemorrhage model revealed a significantly shortened hemostasis time of 132.5 ± 12.82 s for the EDC/NHS cross-linked dressings compared to the gauze in the blank group (hemostasis time of 251.43 ± 10.69 s). These findings indicated that C/SA-based dressings exhibited both good biocompatibility and a significant hemostatic effect, making them suitable for biomedical applications.
摘要：
在我们寻找生物相容性复合止血敷料的过程中,我们专注于由两种天然生物成分组成的新型生物材料的设计，胶原蛋白和海藻酸钠（SA），使用1-乙基-3-(3-二甲基氨基丙基)碳二亚胺/N-羟基琥珀酰亚胺(EDC/NHS)和氧化藻酸钠(OSA)交联。我们进行了一系列测试，以评估其理化性质，急性全身毒性，皮肤刺激,皮内反应,致敏,细胞毒性，和体内股动脉出血模型。结果证明了胶原/海藻酸钠(C/SA)基敷料在交联前后具有优异的生物相容性。具体来说,股动脉出血模型显示,EDC/NHS交联敷料与空白组纱布相比,止血时间显著缩短,为132.5±12.82s(止血时间为251.43±10.69s).这些发现表明，基于C/SA的敷料具有良好的生物相容性和显着的止血效果。使它们适合生物医学应用。
