PDF(Pubmed)
Abstract：
Background: This cross-sectional study aimed to identify the prevalence of Candida albicans and Malassezia globosa in children with severe early childhood caries and caries-free children in Hong Kong. Methods: This study first recruited a total of 80 children aged between 48 and 72 months old, 40 children with severe early childhood caries, and 40 caries-free children. The children were then further divided into four groups, with 20 children in each group: Group 1: Severe early childhood caries-C. albicans, Group 2: Severe early childhood caries-M. globosa, Group 3: Caries-free-C. albicans and Group 4: Caries-free-M. globosa. Saliva, plaque, and caries lesion samples were collected from participants with severe early childhood caries, while only saliva and plaque samples were collected from caries-free participants. Caries status of the primary molars was assessed using WHO\'s decayed, missing, and filled tooth index, and the severity of cavitated lesions was determined based on International Caries Diagnosis and Assessment System criteria as caries code 5 or 6. The samples were analyzed using an Internal Transcribed Space and Quantitative Real-Time Polymerase Chain Reaction. Results:C. albicans was more prevalent in saliva and plaque samples of severe early childhood caries than in the caries-free group. Proportion of C. albicans in both saliva and plaque samples differed significantly between severe early childhood caries and caries-free groups (p < 0.05). Within the severe early childhood caries group, the proportion of children with C. albicans varied between 6 and 46%. No significant difference in M. globosa load was found between plaque samples of the severe early childhood caries and caries-free groups (p = 0.159). Conversely, no significant difference in M. globosa load was observed between saliva samples of severe early childhood caries and caries-free groups (p = 0.051). Conclusions: This study demonstrated a strong association between C. albicans and severe early childhood caries. M. globosa was detected in both the caries-free and severe early childhood caries groups, albeit at low levels.
摘要：
背景：这项横断面研究旨在确定香港患有严重早期儿童龋齿和无龋齿儿童的白色念珠菌和球形马拉色菌的患病率。方法：本研究首先招募了80名年龄在48至72个月之间的儿童，40名儿童患有严重的早期龋齿，和40个没有龋齿的孩子。然后孩子们被进一步分成四组，每组20名儿童：第1组：严重的儿童早期龋齿。白色念珠菌,第2组：严重的儿童早期龋齿-M.globosa,第三组：无龋齿C.白色念珠菌和第4组：无龋齿-M.globosa.唾液,牌匾,龋齿病变样本是从患有严重儿童早期龋齿的参与者那里收集的，而仅从无龋齿参与者收集唾液和斑块样本。原发性磨牙的龋齿状况是使用世界卫生组织的腐烂进行评估的，失踪,填充牙齿指数，根据国际龋齿诊断和评估系统标准5或6确定空化病变的严重程度。使用内部转录空间和定量实时聚合酶链反应分析样品。结果：C.白色念珠菌在严重早期儿童龋齿的唾液和牙菌斑样本中比在无龋齿组中更为普遍。白色念珠菌在唾液和菌斑样本中的比例在重度早期儿童龋齿和无龋齿组之间显著不同(p<0.05)。在严重的儿童早期龋齿组中，患有白色念珠菌的儿童比例在6%到46%之间。在严重的早期儿童龋齿和无龋齿组的菌斑样本之间，球藻负荷没有显着差异（p=0.159）。相反，在严重的早期儿童龋齿组和无龋齿组的唾液样本之间，未观察到球形支原体负荷的显着差异（p=0.051）。结论：这项研究表明白色念珠菌与严重的儿童早期龋齿之间有很强的关联。在无龋齿和严重的早期儿童龋齿组中都检测到球形分枝杆菌，尽管处于低水平。
