PDF(Pubmed)
Abstract：
Highly sensitive and specific biomarker detection is of outstanding importance for the diagnosis and treatment of cancers. Herein, we developed robust photoelectrochemical (PEC) biosensors with low background noise and high sensitivity based on a heterojunction, which can improve semiconductor photoelectric properties by limiting the recombination of photogenerated electron-hole pairs and successfully widening the range of light absorption. Alpha-fetoprotein (AFP) was used as a target model to examine the analytical performances of the designed PEC biosensors. ZnO/Cs3MnBr5 heterogeneous film with a uniform porous structure and large surface area enhanced electron transfer and biomolecule immobilization, and significantly increased the photocurrent response. Under the optimal conditions, the designed PEC biosensor exhibited a linear detection range of 0.01-500 ng/mL and a detection limit of 12 pg/mL. In addition, this PEC biosensor performed well when testing human serum samples and exhibited good repeatability, stability over time, and specificity, showing enormous potential for the detection of cancer markers in future biological and clinical research.
摘要：
高灵敏度和特异性的生物标志物检测对于癌症的诊断和治疗非常重要。在这里，我们开发了基于异质结的低背景噪声和高灵敏度的鲁棒光电化学（PEC）生物传感器，可以通过限制光生电子-空穴对的复合和成功地扩大光吸收范围来提高半导体的光电性能。甲胎蛋白（AFP）用作目标模型，以检查设计的PEC生物传感器的分析性能。ZnO/Cs3MnBr5异质薄膜具有均匀的多孔结构和大的比表面积，增强了电子转移和生物分子固定，并显著增加了光电流响应。在最优条件下,设计的PEC生物传感器的线性检测范围为0.01-500ng/mL，检出限为12pg/mL。此外,这种PEC生物传感器在测试人血清样品时表现良好，并表现出良好的可重复性，随着时间的推移稳定性，和特异性，在未来的生物学和临床研究中显示出检测癌症标志物的巨大潜力。
