PDF(Pubmed)
Abstract：
This study investigates the valorization potential of yellowfin tuna (Thunnus albacares) tails to produce high-value commercial products. Firstly, the tuna tails were placed in a perforated stainless-steel cylinder, and hydraulic pressure was applied to separate the skin from the muscle in the tails. The extracted muscle was then utilized as a nitrogen source for the growth of the proteolytic enzyme producer Bacillus subtilis, while the skins were employed for gelatin extraction. The proteases from B. subtilis were partially purified and used to produce antioxidant peptides from the obtained gelatin. The gelatin formed a gel upon cooling, with gelling and melting temperatures of 16 °C and 22 °C, respectively, and a Bloom strength of approximately 160. Response Surface Methodology (RSM) was employed to determine the optimal hydrolysis conditions to achieve the highest antioxidant activity (35.96% measured as DPPH radical scavenging activity), which were 50 °C and 6.5 IU of enzyme. The findings emphasize the importance of an integrated approach to maximize the value of tuna by-products, promoting sustainability within the framework of a circular bioeconomy. Overall, these results contribute to the efficient utilization of tuna by-products, waste reduction, and enhanced economic viability of the tuna industry.
摘要：
这项研究调查了黄鳍金枪鱼（Thunnusalbacares）尾巴的增值潜力，以生产高价值的商业产品。首先,金枪鱼尾巴被放置在穿孔的不锈钢圆筒中，并施加液压以将皮肤与尾巴中的肌肉分开。然后将提取的肌肉用作蛋白水解酶生产者枯草芽孢杆菌生长的氮源，而皮肤用于明胶提取。将来自枯草芽孢杆菌的蛋白酶部分纯化并用于从获得的明胶生产抗氧化肽。明胶冷却后形成凝胶，胶凝和熔融温度为16°C和22°C，分别,布卢姆强度约为160。响应面法（RSM）用于确定最佳水解条件，以实现最高的抗氧化活性（35.96％作为DPPH自由基清除活性测量），这是50°C和6.5IU的酶。研究结果强调了采取综合方法最大限度地提高金枪鱼副产品价值的重要性,在循环生物经济框架内促进可持续性。总的来说,这些结果有助于金枪鱼副产品的有效利用，减少废物，并提高金枪鱼产业的经济可行性。
