PDF(Pubmed)
Abstract：
Haskap berry (Lonicera caerulea L.) is a rich dietary source of anthocyanins with potent anti-inflammatory properties. In this study, isolated haskap berry anthocyanins were encapsulated in maltodextrin and inulin (3:1) by freeze-drying to improve stability and bioavailability. The structural properties of microcapsules, encapsulation yield, efficiency, recovery, and powder retention were evaluated. The microcapsules that exhibited the highest encapsulation efficiency (60%) and anthocyanin recovery (89%) were used in the dextran sulfate sodium (DSS)-induced acute colitis in mice. Thirty-five BALB/c male mice of seven weeks old were divided into seven dietary supplementation groups (n = 5) to receive either free anthocyanins, encapsulated anthocyanins (6.2 mg/day), or probiotics (1 × 109 CFU/day) alone or as combinations of anthocyanin and probiotics. As observed by clinical data, free anthocyanin and probiotic supplementation significantly reduced the severity of colitis. The supplementary diets suppressed the DSS-induced elevation of serum inflammatory (interleukin (IL)-6 and tumor necrosis factor) and apoptosis markers (B-cell lymphoma 2 and Bcl-2-associated X protein) in mice colon tissues. The free anthocyanins and probiotics significantly reduced the serum IL-6 levels. In conclusion, the dietary supplementation of haskap berry anthocyanins and probiotics protects against DSS-induced colitis possibly by attenuating mucosal inflammation, and this combination has the potential as a health-promoting dietary supplement and nutraceutical.
摘要：
Haskap浆果（LoniceracaeruleaL.）是花青素的丰富饮食来源，具有有效的抗炎特性。在这项研究中,通过冷冻干燥将分离的haskap浆果花色苷包封在麦芽糊精和菊粉（3：1）中，以提高稳定性和生物利用度。微胶囊的结构特性,包封率，效率,recovery,和粉末保留进行评估。在葡聚糖硫酸钠（DSS）诱导的小鼠急性结肠炎中使用了具有最高包封效率（60％）和花色苷回收率（89％）的微胶囊。将35只7周龄的BALB/c雄性小鼠分为7个饮食补充组（n=5），接受游离花青素，包封的花色苷（6.2毫克/天），或单独的益生菌（1×109CFU/天）或作为花青素苷和益生菌的组合。根据临床数据观察，游离花青素和益生菌补充剂显著降低结肠炎的严重程度。补充饮食抑制了DSS诱导的小鼠结肠组织中血清炎症（白介素（IL）-6和肿瘤坏死因子）和凋亡标志物（B细胞淋巴瘤2和Bcl-2相关X蛋白）的升高。游离花色苷和益生菌显著降低血清IL-6水平。总之,Haskap浆果花青素和益生菌的饮食补充可能通过减轻粘膜炎症来保护DSS诱导的结肠炎，这种组合具有作为促进健康的膳食补充剂和营养保健品的潜力。
