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Abstract：
In this present study, bioinformatics analysis and the experimental validation method were used to systematically explore the antioxidant activity and anti-inflammatory effect of Lactiplantibacillus plantarum A106, which was isolated from traditional Chinese pickles, on lipopolysaccharide (LPS)-induced RAW264.7 macrophages. L. plantarum A106 had a good scavenging ability for DPPH, ABTS, and hydroxyl radicals. Furthermore, L. plantarum A106 could increase the activity of RAW264.7 macrophages; raise the SOD and GSH levels, with or without LPS sensitization; or decrease the MDA, TNF-α, and IL-6 levels. In order to deeply seek the antioxidant and anti-inflammatory role and mechanism, bioinformatic analysis, including GO, KEGG, and GSEA analysis, was used to conduct an in-depth analysis, and the results showed that the LPS treatment of RAW264.7 macrophages significantly upregulated inflammatory-related genes and revealed an enrichment in the inflammatory signaling pathways. Additionally, a network analysis via the Cytoscape software (version 3.9.1) identified key central genes and found that LPS also disturbed apoptosis and mitochondrial function. Based on the above bioinformatics analysis, the effects of L. plantarum A106 on inflammation-related gene expression, mitochondrial function, apoptosis, etc., were detected. The results indicated that L. plantarum A106 restored the declined expression levels of crucial genes like TNF-α and IL-6; mitochondrial membrane potential; and apoptosis and the expression of apoptosis-related genes, Bcl-2, Caspase-3, and Bax. These results suggest that L. plantarum A106 exerts antioxidant activity and anti-inflammatory effects through regulating inflammatory and apoptosis-related gene expression, restoring the mitochondrial membrane potential.
摘要：
在本研究中,采用生物信息学分析和实验验证方法，系统探索了从中国传统泡菜中分离的植物乳杆菌A106的抗氧化活性和抗炎作用，脂多糖（LPS）诱导的RAW264.7巨噬细胞。植物乳杆菌A106对DPPH具有良好的清除能力，ABTS,和羟基自由基。此外，植物乳杆菌A106可以增加RAW264.7巨噬细胞的活性；提高SOD和GSH水平，有或没有LPS致敏；或降低MDA，TNF-α,和IL-6水平。为了深入寻求其抗氧化和抗炎的作用和机制,生物信息学分析，包括GO,KEGG,和GSEA分析，被用来进行深入分析，结果表明，LPS处理RAW264.7巨噬细胞显著上调炎症相关基因，揭示了炎症信号通路的富集。此外,通过Cytoscape软件（3.9.1版）进行的网络分析确定了关键的中心基因，并发现LPS也会干扰细胞凋亡和线粒体功能。基于以上生物信息学分析,植物乳杆菌A106对炎症相关基因表达的影响，线粒体功能，凋亡，等。,被检测到。结果表明，植物乳杆菌A106恢复了TNF-α和IL-6等关键基因的表达水平下降；线粒体膜电位；凋亡和凋亡相关基因的表达。Bcl-2、Caspase-3和Bax。这些结果表明，植物乳杆菌A106通过调节炎症和凋亡相关基因表达发挥抗氧化活性和抗炎作用。恢复线粒体膜电位.
