PDF(Pubmed)
Abstract：
Intestinal infections caused by Escherichia coli and Salmonella enterica pose a huge economic burden on the swine industry that is exacerbated by the development of antimicrobial resistance in these pathogens, thus raising the need for alternative prevention and treatment methods. Our aim was to test the beneficial effects of the flavonoid luteolin in an in vitro model of porcine intestinal infections. We infected the porcine intestinal epithelial cell line IPEC-J2 with E. coli and S. enterica subsp. enterica serovar Typhimurium (106 CFU/mL) with or without previous, concurrent, or subsequent treatment with luteolin (25 or 50 µg/mL), and measured the changes in the reactive oxygen species and interleukin-6 and -8 levels of cells. We also tested the ability of luteolin to inhibit the adhesion of bacteria to the cell layer, and to counteract the barrier integrity damage caused by the pathogens. Luteolin was able to alleviate oxidative stress, inflammation, and barrier integrity damage, but it could not inhibit the adhesion of bacteria to IPEC-J2 cells. Luteolin is a promising candidate to be used in intestinal infections of pigs, however, further studies are needed to confirm its efficacy. The use of luteolin in the future could ultimately lead to a reduced need for antibiotics in pig production.
摘要：
由大肠杆菌和沙门氏菌引起的肠道感染对养猪业构成了巨大的经济负担，这些病原体的抗菌素耐药性的发展加剧了这种负担。从而提高了对替代预防和治疗方法的需求。我们的目的是在猪肠道感染的体外模型中测试类黄酮木犀草素的有益作用。我们用大肠杆菌和肠链球菌亚种感染猪肠上皮细胞系IPEC-J2。肠鼠伤寒血清型（106CFU/mL），有或没有以前的，并发,或随后用木犀草素（25或50µg/mL）处理，并测量了细胞的活性氧和白细胞介素-6和-8水平的变化。我们还测试了木犀草素抑制细菌粘附于细胞层的能力，并抵消病原体造成的屏障完整性损害。木犀草素能够缓解氧化应激，炎症,和屏障完整性损坏，但不能抑制细菌对IPEC-J2细胞的粘附。木犀草素是一种有希望用于猪肠道感染的候选药物，然而,需要进一步的研究来证实其疗效.未来使用木犀草素可能最终导致猪生产中对抗生素的需求减少。
