PDF(Pubmed)
Abstract：
The common carp (Cyprinus carpio) is one of the most important aquaculture species in China, known for its remarkable adaptability and nutritional profile. However, the specific molecular response mechanisms regulating the nutritional deposition of carp remain inadequately elucidated. This study conducted a comprehensive analysis of muscle nutritional content and transcriptome data from liver and muscle tissues of three distinct carp varieties. The aim was to elucidate the key genes and signaling pathways that regulate muscle nutritional composition in carp. The findings revealed that FFRC carp (FFRC) exhibited significantly higher levels of crude fat, total n-3 polyunsaturated fatty acids, and total n-6 polyunsaturated fatty acids in muscle tissue compared to Ying carp (YC) and Huanghe carp (HC) (p < 0.05). Transcriptomic analyses correlated these elevated levels with a marked upregulation of genes involved in the activation and transportation of fatty acid (fabp7, acsl5, acsbg2) as well as biosynthesis and elongation of long-chain unsaturated fatty acids (elovl2, fads2) within the liver. Furthermore, the flavor amino acid, essential amino acids, and crude protein content in the muscle of HC were significantly higher than in FFRC and YC (p < 0.05). Transcriptomic analyses indicated that this was associated with significant changes in the expression of genes related to amino acid metabolism (asns, alt, ldha, glul, setd, prodh, l3hypdh, hoga1) within their muscle tissue. This research provides a theoretical foundation for the precise modulation of the muscle nutritional composition in carp.
摘要：
鲤鱼(Cyprinuscarpio)是我国重要的水产养殖品种之一,以其卓越的适应性和营养特征而闻名。然而,调节鲤鱼营养沉积的特定分子反应机制仍未充分阐明。这项研究对三个不同鲤鱼品种的肝脏和肌肉组织的肌肉营养成分和转录组数据进行了综合分析。旨在阐明调节鲤鱼肌肉营养成分的关键基因和信号通路。研究结果表明，FFRC鲤鱼（FFRC）表现出明显较高的粗脂肪水平，总n-3多不饱和脂肪酸，和肌肉组织中总n-6多不饱和脂肪酸与营鱼（YC）和黄河鱼（HC）相比（p<0.05）。转录组学分析将这些升高的水平与参与脂肪酸（fabp7，acsl5，acsbg2）的激活和运输以及长链不饱和脂肪酸（elovl2，fads2）的生物合成和延伸的基因的显着上调相关联。肝脏。此外，风味氨基酸，必需氨基酸,HC肌肉中的粗蛋白含量明显高于FFRC和YC（p<0.05）。转录组分析表明，这与氨基酸代谢相关基因表达的显着变化有关（asns，alt,ldha,glul,setd,prodh,l3hypdh,hoga1）在他们的肌肉组织内。本研究为精确调控鲤鱼肌肉营养成分提供了理论依据。
