PDF(Pubmed)
Abstract：
The aim of this study was to explore alterations in plasma metabolites among mares afflicted with endometritis. Mares were divided into two groups, namely, the equine endometritis group (n = 8) and the healthy control group (n = 8), which included four pregnant and four non-pregnant mares, using a combination of clinical assessment and laboratory confirmation. Plasma samples from both groups of mares were analyzed through untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolomics. A total of 28 differentially abundant metabolites were identified by screening and identifying differentially abundant metabolites and analyzing the pathway enrichment of differentially. Ten metabolites were identified as potential biomarkers for the diagnosis of endometritis in mares. Among them, seven exhibited a decrease in the endometritis groups, including hexadecanedioic acid, oleoyl ethanolamide (OEA), [fahydroxy(18:0)]12_13-dihydroxy-9z-octa (12,13-diHOME), deoxycholic acid 3-glucuronide (DCA-3G), 2-oxindole, and (+/-)9-HPODE, and 13(S)-HOTRE. On the other hand, three metabolites, adenosine 5\'-monophosphate (AMP), 5-hydroxy-dl-tryptophan (5-HTP), and l-formylkynurenine, demonstrated an increase. These substances primarily participate in the metabolism of tryptophan and linolenic acid, as well as fat and energy. In conclusion, metabolomics revealed differentially abundant metabolite changes in patients with mare endometritis. These specific metabolites can be used as potential biomarkers for the non-invasive diagnosis of mare endometritis.
摘要：
这项研究的目的是探索患有子宫内膜炎的母马中血浆代谢物的变化。Mares被分成两组,即,马子宫内膜炎组(n=8)和健康对照组(n=8),其中包括四只怀孕的母马和四只未怀孕的母马，结合使用临床评估和实验室确认。通过非靶向液相色谱-串联质谱（LC-MS/MS）代谢组学分析两组母马的血浆样品。通过筛选和鉴定差异丰富代谢物并分析差异富集途径，共鉴定了28种差异丰富代谢物。十种代谢物被鉴定为诊断母马子宫内膜炎的潜在生物标志物。其中,七个子宫内膜炎组表现出减少，包括十六烷二酸,油酰乙醇酰胺（OEA），[fahydroxid(18:0)]12_13-二羟基-9z-octa(12,13-diHOME),脱氧胆酸3-葡糖苷酸（DCA-3G），2-羟吲哚，和（+/-）9-HPODE，和13(S)-HOTRE。另一方面,三种代谢物，5'-单磷酸腺苷（AMP），5-羟基-dl-色氨酸（5-HTP），和L-甲酰基犬尿氨酸,显示增加。这些物质主要参与色氨酸和亚麻酸的代谢，以及脂肪和能量。总之,代谢组学揭示了母马子宫内膜炎患者的代谢产物差异丰富.这些特异性代谢产物可作为潜在的生物标志物用于母马子宫内膜炎的非侵入性诊断。
