Abstract:
Trichomonas gallinae (T. gallinae) is a flagellated protozoan and the causative agent of trichomoniasis, or canker, in birds. In the current study, the prevalence of T. gallinae was firstly investigated in five breeds. According to the results of the prevalence study, White King pigeons were selected as the experimental animals. A total of 135 White King squabs at one day of age were randomly divided into two groups and raised in separate isolators. The challenged group (N = 100) was challenged intranasally with 5 × 106 parasites/mL of the T. gallinae strain, and the control group (N = 35) was intranasally administered medium of equivalent volume. At 1, 2, 3 and 5 days post infection (DPIs), the crops and esophagi were collected for RNA extraction and formaldehyde fixation. The results showed that prevalence of T. gallinae in the five breeds ranged from 27.13% (White Carneau) to 43.14% (White King). After the challenge, mild microscopic lesions were observed in both tissues. Apoptosis rates were higher in the challenged group than in the control group at 2 and 5 DPIs in the crop and at 1, 2 and 7 DPIs in the esophagus. For both tissues, relative expression of IL-1β increased dramatically at the beginning and decreased at 5 DPIs, and TGF-β increased stably in the challenged group.
摘要:
鸡毛滴虫(T.gallinae)是鞭毛的原生动物,是毛滴虫病的病原体,或溃疡,在鸟。在目前的研究中,首次在5个品种中调查了鸡鸡的流行情况。根据患病率研究的结果,选择白王鸽子作为实验动物。将一天大的总共135个白色国王的乳鼠随机分为两组,并在单独的隔离器中饲养。攻击组(N=100)鼻内攻击5×106寄生虫/mL的鸡木霉菌株,对照组(N=35)鼻内给药等体积培养基。在感染后1、2、3和5天(DPI),收集作物和食道进行RNA提取和甲醛固定。结果表明,五个品种中的胆虫患病率从27.13%(WhiteCarneau)到43.14%(WhiteKing)。挑战之后,在两个组织中均观察到轻度的微观病变。在作物中2和5个DPI以及食道中1、2和7个DPI时,攻击组的凋亡率高于对照组。对于这两种组织,IL-1β的相对表达在开始时急剧增加,在5个DPI时降低,和TGF-β在攻击组中稳定增加。