Abstract:
This study aimed to determine whether the eggs of laying hens fed caffeine contain this compound and its primary metabolites (theophylline, theobromine, and paraxanthine). Laying hens were distributed into four experimental groups fed rations containing 0 (control), 150, 300, or 450 μg/g of caffeine. For residual analysis, six eggs per group were collected after 4, 8, and 12 weeks. The concentrations of caffeine, theophylline, theobromine, and paraxanthine were determined in the white and yolk of each egg by a high-performance liquid chromatography with photodiode array detector (HPLC-PDA) method. All four compounds were detected in the white and yolk of eggs produced by hens fed caffeine, but their levels in the egg white were approximately twice those in the yolk. The major metabolite found in eggs was theophylline (57.5% of caffeine metabolites in the egg white and 58.5% in the yolk), followed by theobromine (39.9% in the egg white and 41.5% in the yolk), and paraxanthine (2.64% in the egg white and non-detected in the yolk). In summary, caffeine and its metabolites, theophylline, theobromine, and paraxanthine, are transferred to the chicken eggs.
摘要:
这项研究旨在确定饲喂咖啡因的蛋鸡的卵是否含有这种化合物及其主要代谢产物(茶碱,可可碱,和副黄嘌呤)。产蛋鸡分为四个实验组,饲喂含有0(对照)的口粮,150、300或450μg/g咖啡因。对于残差分析,在第4、8和12周之后,每组收集6个卵。咖啡因的浓度,茶碱,可可碱,用高效液相色谱-光电二极管阵列检测器(HPLC-PDA)法测定每个鸡蛋的蛋黄和蛋黄中的黄嘌呤。在饲喂咖啡因的母鸡生产的蛋黄和蛋黄中检测到所有四种化合物,但是它们在蛋清中的含量大约是蛋黄中的两倍。在鸡蛋中发现的主要代谢产物是茶碱(在蛋清中占咖啡因代谢产物的57.5%,在蛋黄中占58.5%),其次是可可碱(蛋清中39.9%,蛋黄中41.5%),和对黄嘌呤(蛋清中2.64%,蛋黄中未检测到)。总之,咖啡因及其代谢产物,茶碱,可可碱,和副黄嘌呤,被转移到鸡蛋上。