METHODS: We used interleukin (IL)-1β stimulation to establish an NPC-degenerated IVDD model and investigated whether human urine-derived stem cell (USC) exosomes could prevent IL-1β-induced NPC degeneration using western blotting, quantitative real-time polymerase chain reaction, flow cytometry, and transcriptome sequencing techniques.
RESULTS: We successfully extracted and identified USCs and exosomes from human urine. IL-1β substantially downregulated NPC viability and induced NPC degeneration while modulating the expression of SOX-9, collagen II, and aggrecan. Exosomes from USCs could rescue IL-1β-induced NPC degeneration and restore the expression levels of SOX-9, collagen II, and aggrecan.
CONCLUSIONS: USC-derived exosomes can prevent NPCs from degeneration following IL-1β stimulation. This finding can aid the development of a potential treatment strategy for IVDD.
方法:我们使用白细胞介素(IL)-1β刺激建立NPC退化的IVDD模型,并使用蛋白质印迹法研究人尿源干细胞(USC)外泌体是否可以预防IL-1β诱导的NPC变性,定量实时聚合酶链反应,流式细胞术,和转录组测序技术。
结果:我们成功地从人尿液中提取并鉴定了USCs和外泌体。IL-1β显著下调NPC活力并诱导NPC变性,同时调节SOX-9,胶原II的表达,和aggrecan。来自USCs的外泌体可以挽救IL-1β诱导的NPC变性并恢复SOX-9,胶原蛋白II的表达水平,和aggrecan。
结论:USC衍生的外泌体可以防止IL-1β刺激后NPC变性。这一发现可以帮助开发IVDD的潜在治疗策略。