Abstract：
OBJECTIVE: This study aimed to investigate the impact of hepatocyte growth factor (HGF) on colonic morphology and gut microbiota in a rat model of short bowel syndrome (SBS).
METHODS: SD rats underwent jugular vein catheterization for total parenteral nutrition (TPN) and 90% small bowel resection [TPN + SBS (control group) or TPN + SBS + intravenous HGF (0.3 mg/kg/day, HGF group)]. Rats were harvested on day 7. Colonic morphology, gut microflora, tight junction, and Toll-like receptor-4 (TLR4) were evaluated.
RESULTS: No significant differences were observed in the colonic morphological assessment. No significant differences were observed in the expression of tight junction-related genes in the proximal colon. However, the claudin-1 expression tended to increase and the claudin-3 expression tended to decrease in the distal colon of the HGF group. The Verrucomicrobiota in the gut microflora of the colon tended to increase in the HGF group. The abundance of most LPS-producing microbiota was lower in the HGF group than in the control group. The gene expression of TLR4 was significantly downregulated in the distal colon of the HGF group.
CONCLUSIONS: HGF may enhance the mucus barrier through the tight junctions or gut microbiome in the distal colon.
METHODS: SD rats underwent jugular vein catheterization for total parenteral nutrition (TPN) and 90% small bowel resection [TPN + SBS (control group) or TPN + SBS + intravenous HGF (0.3 mg/kg/day, HGF group)]. Rats were harvested on day 7. Colonic morphology, gut microflora, tight junction, and Toll-like receptor-4 (TLR4) were evaluated.
RESULTS: No significant differences were observed in the colonic morphological assessment. No significant differences were observed in the expression of tight junction-related genes in the proximal colon. However, the claudin-1 expression tended to increase and the claudin-3 expression tended to decrease in the distal colon of the HGF group. The Verrucomicrobiota in the gut microflora of the colon tended to increase in the HGF group. The abundance of most LPS-producing microbiota was lower in the HGF group than in the control group. The gene expression of TLR4 was significantly downregulated in the distal colon of the HGF group.
CONCLUSIONS: HGF may enhance the mucus barrier through the tight junctions or gut microbiome in the distal colon.
摘要：
目的：本研究旨在探讨肝细胞生长因子（HGF）对短肠综合征（SBS）大鼠结肠形态和肠道菌群的影响。
方法：SD大鼠进行颈静脉置管，进行全胃肠外营养（TPN）和90％小肠切除术[TPNSBS（对照组）或TPNSBS静脉HGF（0.3mg/kg/天，HGF组)]。在第7天收获大鼠。结肠形态,肠道菌群，紧密连接,和Toll样受体4（TLR4）进行评估。
结果:在结肠形态学评估中没有观察到显著差异。近端结肠中紧密连接相关基因的表达没有观察到显着差异。然而,在HGF组的远端结肠中，claudin-1表达趋于增加，而claudin-3表达趋于减少。在HGF组中，结肠肠道微生物区系中的Verrucomicrobiota倾向于增加。HGF组中大多数产生LPS的微生物群的丰度低于对照组。HGF组远端结肠TLR4基因表达显著下调。
结论：HGF可能通过远端结肠的紧密连接或肠道微生物组增强粘液屏障。
方法：SD大鼠进行颈静脉置管，进行全胃肠外营养（TPN）和90％小肠切除术[TPNSBS（对照组）或TPNSBS静脉HGF（0.3mg/kg/天，HGF组)]。在第7天收获大鼠。结肠形态,肠道菌群，紧密连接,和Toll样受体4（TLR4）进行评估。
结果:在结肠形态学评估中没有观察到显著差异。近端结肠中紧密连接相关基因的表达没有观察到显着差异。然而,在HGF组的远端结肠中，claudin-1表达趋于增加，而claudin-3表达趋于减少。在HGF组中，结肠肠道微生物区系中的Verrucomicrobiota倾向于增加。HGF组中大多数产生LPS的微生物群的丰度低于对照组。HGF组远端结肠TLR4基因表达显著下调。
结论：HGF可能通过远端结肠的紧密连接或肠道微生物组增强粘液屏障。
