Abstract：
Adipose tissue is recognized not only as an endocrine organ but also as a reservoir for adipose-derived stromal/stem cells (ASCs). ASCs have stimulated the interest of both the scientific and medical communities due to their therapeutic potential and applications in tissue engineering and regenerative medicine. ASCs are leveraged for their multipotency and their paracrine function. ASC behavior is highly variable and donor dependent. Donor age, body mass index, disease status, sex, and ethnicity can lead to differential overall function and quality. The impact of donor age and passage on ASC behavior has been well documented, impacting cell proliferation and differentiation potential and thus must be taken into careful consideration when conducting in vitro studies. Pooling of ASCs from different donors reduces heterogeneity among individual donors and produces ASCs with a consistent differentiation and paracrine profiles, an advantage for studies in biological aging. This chapter provides a detailed overview for studies related to quality control for ASC pools considering biological and chronological aging in ASCs. There are hallmarks of biological aging and specific assays associated with the evaluation of each hallmark. Nevertheless, here we present the assays that provide a standardized characterization and qualification of donor pools for their regenerative potential, considering chronological and biological age of the pool. The assays included in this chapter are considered quality control standards to evaluate cell proliferation, differentiation, colony-forming units, and cellular senescence from different donor age and cell passage cohorts.
摘要：
脂肪组织不仅被认为是内分泌器官，而且被认为是脂肪来源的基质/干细胞（ASC）的储库。由于ASC的治疗潜力和在组织工程和再生医学中的应用，ASC激发了科学界和医学界的兴趣。ASC利用其多能性和旁分泌功能。ASC行为是高度可变的并且依赖于供体。捐赠者年龄,身体质量指数,疾病状态,性别,和种族会导致整体功能和质量的差异。供体年龄和通道对ASC行为的影响已经有据可查，影响细胞增殖和分化潜力，因此在进行体外研究时必须仔细考虑。来自不同供体的ASC的汇集减少个体供体之间的异质性,并产生具有一致的分化和旁分泌谱的ASC。生物衰老研究的优势。本章为ASC池的质量控制相关研究提供了详细的概述，这些研究考虑了ASC中的生物学和时间老化。存在生物老化的标志和与每个标志的评估相关的特定测定。然而，在这里，我们介绍了提供供体库再生潜力的标准化表征和资格鉴定的测定方法，考虑到游泳池的时间和生物年龄。本章中包含的分析被认为是评估细胞增殖的质量控制标准，分化,菌落形成单位，和来自不同供体年龄和细胞传代队列的细胞衰老。
