Abstract：
Peptide antibodies are particularly useful for immunocytochemistry (ICC) and immunohistochemistry (IHC), where antigens may denature due to fixation of tissues and cells. Peptide antibodies can be made to any defined sequence, including unknown putative proteins and posttranslationally modified sequences. Moreover, the availability of large amounts of the antigen (peptide) allows inhibition/absorption controls, which are important in ICC/IHC, due to the many possibilities for false-positive reactions caused by immunoglobulin Fc receptors, nonspecific reactions and cross-reactivity of primary and secondary antibodies with other antigens and endogenous immunoglobulins, respectively. Here, simple protocols for ICC and IHC are described together with recommendations for appropriate controls.
摘要：
肽抗体对于免疫细胞化学（ICC）和免疫组织化学（IHC）特别有用，由于组织和细胞的固定，抗原可能会变性。肽抗体可以制成任何定义的序列，包括未知的推定蛋白质和翻译后修饰的序列。此外，大量抗原（肽）的可用性允许抑制/吸收控制，这在ICC/IHC中很重要，由于免疫球蛋白Fc受体引起的假阳性反应的许多可能性，一级和二级抗体与其他抗原和内源性免疫球蛋白的非特异性反应和交叉反应性，分别。这里,介绍了ICC和IHC的简单协议以及适当控制的建议.
