Abstract：
Enzyme-linked immunosorbent assay (ELISA) detects qualitatively and quantitatively the presence of antibodies or antigens in a sample. Due to its simplicity, high sensitivity, and user-friendliness, the test is widely used in laboratory research, clinical diagnoses, and food testing. This chapter describes the indirect semiquantitative ELISA protocol used to monitor antibody levels in animals and analyze the titer levels of specific antibodies against a target antigen in serum and saliva.
摘要：
酶联免疫吸附测定(ELISA)定性和定量地检测样品中抗体或抗原的存在。由于其简单性，高灵敏度,和用户友好性，该测试广泛用于实验室研究，临床诊断,和食物测试。本章介绍了用于监测动物抗体水平和分析血清和唾液中针对靶抗原的特异性抗体的滴度水平的间接半定量ELISA方案。
