关键词: Bleeding Coagulation Goats Horses and chickens Immunization Immunoassays Mice Peptide antisera Pigs Polyclonal antibodies Rabbits Serum Sheep

Mesh : Animals Immune Sera / chemistry immunology Mice Rabbits Peptides / immunology Immunization Horses / immunology Sheep Goats Swine Chickens / immunology

来  源:   DOI:10.1007/978-1-0716-3914-6_10

Abstract:
Polyclonal antibodies are relatively easy to produce and may supplement monoclonal antibodies for some applications or even have some advantages.The choice of species for production of (peptide) antisera is based on practical considerations, including availability of immunogen (vaccine) and animals. Two major factors govern the production of antisera: the nature of adaptive immune responses, which take place over days/weeks and ethical guidelines for animal welfare.Here, simple procedures for immunization of mice, rabbits, sheep, goats, pigs, horses, and chickens are presented.
摘要:
多克隆抗体相对容易产生,并且可以补充单克隆抗体用于一些应用或甚至具有一些优点。用于生产(肽)抗血清的物种的选择基于实际考虑,包括免疫原(疫苗)和动物的可用性。两个主要因素控制着抗血清的产生:适应性免疫反应的性质,这发生在几天/几周和动物福利的道德准则。这里,小鼠免疫接种的简单程序,兔子,绵羊,山羊,猪,马,和鸡呈现。
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