PDF(Pubmed)
Abstract：
Small non-coding RNAs (sncRNAs) make up ~1% of the transcriptome; nevertheless, they play significant roles in regulating cellular processes. Given the complexity of the central nervous system, sncRNAs likely hold particular importance in the human brain. In this study, we provide sncRNA transcriptomic profiles in a range of adult and prenatal brain regions, with a focus on piRNAs, due to their underexplored expression in somatic cells and tissue-specific nature. Using the WIND workflow, which combines two detection methods, we found 1333 (731 miRNAs, 249 piRNAs, 285 snoRNAs, and 68 other sncRNAs) and 1445 unique sncRNAs (770 miRNAs, 307 piRNAs, 289 snoRNAs, and 79 other sncRNAs) in developing and adult brains, respectively. Significant variations were found upon comparison of fetal and adult brain groups, with 82 miRNAs, 17 piRNAs, and 70 snoRNAs enriched in fetal brains and 22 miRNAs, 11 piRNAs in adult brains. This dataset represents a valuable resource for exploring the sncRNA roles in brain function, their involvement in neurological diseases, and the molecular mechanisms behind brain region interactions.
摘要：
小的非编码RNA(sncRNAs)占转录组的约1%；然而，它们在调节细胞过程中起着重要作用。鉴于中枢神经系统的复杂性，sncRNAs可能在人脑中具有特别的重要性。在这项研究中,我们提供了一系列成人和产前大脑区域的sncRNA转录组学图谱，专注于piRNA，由于它们在体细胞中的表达不足和组织特异性。使用WIND工作流程，结合了两种检测方法，我们发现1333(731个miRNAs，249个piRNAs,285个snoRNA，和68个其他sncRNAs）和1445个独特的sncRNAs（770个miRNAs，307个piRNAs,289个snoRNAs，和79个其他sncRNAs）在发育中和成年大脑中，分别。在比较胎儿和成人脑组时发现了显着差异，有82个miRNA，17个piRNAs,和70个富含胎儿大脑的snoRNAs和22个miRNAs，成人大脑中的11个piRNAs。该数据集代表了探索sncRNA在大脑功能中的作用的宝贵资源，他们参与了神经系统疾病，以及大脑区域相互作用背后的分子机制。
