Abstract：
Osteoarthritis (OA) is a common arthritis types in animals that causes persistent pain and reduces quality of life. Although a high-fat diet (HFD) is widely believed to induce obesity and have adverse effects on the body, the connection between HFD and joint health is not well understood. Therefore, in this study, 32 healthy male New Zealand rabbits were randomly divided into four groups: healthy rabbits fed a standard diet (NDG, n=8) or an HFD (HDG, n=8), rabbits fed a standard diet (OAG, n=8) and an HFD (HOG, n=8), and arthritis was induced by intra-articular enzyme injection. After 12 weeks of HFD feeding, articular cartilage, synovium, and subchondral bone were isolated and collected. Joint tissue damage was evaluated using histopathological and imaging tests. The results showed that there was no significant difference in body weight between rabbits fed a normal diet and those fed an HFD. However, the HFD led to an increase in joint injuries in both induced and non-induced arthritis rabbits. Specifically, the HFD induced lipid metabolism disorders and liver damage in vivo, significantly elevating the levels of serum inflammatory cytokines and bone metabolism markers. Moreover, HFD exacerbated articular cartilage damage in the joints and increased the accumulation of inflammatory cells in synovial tissue, resulting in a notable increase in synovial macrophages and inflammatory cytokines. Additionally, HFD accelerated the bone resorption process in subchondral bone, leading to the destruction of bone mass and subchondral bone microstructure. In summary, the results of this study indicate that an HFD can cause histological damage to the articular cartilage, synovium, and subchondral bone in rabbits, exacerbating arthritis in pre-existing joint damage. Notably, weight is not the primary factor in this effect.
摘要：
骨关节炎(OA)是动物中常见的关节炎类型,其引起持续性疼痛并降低生活质量。尽管人们普遍认为高脂饮食（HFD）会诱发肥胖并对身体产生不利影响，尚不清楚HFD与关节健康之间的联系。因此,在这项研究中，32只健康雄性新西兰兔随机分为四组:健康兔饲喂标准饮食(NDG,n=8）或高脂肪饮食（HDG，n=8),饲喂标准饮食的兔子(OAG，n=8）和高脂肪饮食（HOG，n=8),关节内注射酶诱发关节炎。HFD喂养12周后，关节软骨,滑膜,分离并收集软骨下骨。使用组织病理学和影像学测试评估关节组织损伤。结果表明，饲喂正常饮食的兔子与饲喂HFD的兔子之间的体重没有显着差异。然而,高脂饮食导致诱导和非诱导关节炎兔关节损伤增加。具体来说,高脂饮食（HFD）诱导体内脂质代谢紊乱和肝损伤，显着升高血清炎性细胞因子和骨代谢标志物的水平。此外，HFD加重关节软骨损伤，增加滑膜组织中炎性细胞的积累，导致滑膜巨噬细胞和炎性细胞因子显著增加。此外,HFD加速了软骨下骨的骨吸收过程，导致骨量和软骨下骨微结构的破坏。总之,这项研究的结果表明，高脂饮食（HFD）可导致关节软骨的组织学损伤，滑膜,和兔子的软骨下骨，在预先存在的关节损伤中加剧关节炎。值得注意的是,体重不是造成这种影响的主要因素。
