Abstract：
Osteoporotic bone defects are serious medical problems due to their sparse bone structure, difficulty in restoration and reconstruction, and high recurrence rates, which also result in heavy economic and social burdens. Herein, we developed a hierarchical hydrogel composed of alendronate sodium (AS)/Mg2+-loaded inverse opal methylpropenylated gelatin (GelMA) hydrogel microspheres (IOHM-AS-Mgs) within methylpropenylated poly(hyaluronic acid) (HAMA) for osteoporotic bone defect treatment. The IOHM-AS-Mgs displayed good cytocompatibility and cell adhesion and strongly stimulated osteogenesis at the transcriptomic and protein levels. When this treatment was applied to the osteoporotic bone defect area, HAMA was used to fix the microspheres. The results of the microcomputed tomography (micro-CT) and histological analyses indicated that the hierarchical hydrogel had the best therapeutic effect. Therefore, this hydrogel is a new candidate for osteoporotic bone defect treatment.
摘要：
骨质疏松性骨缺损由于其稀疏的骨结构而成为严重的医学问题,恢复和重建的困难，高复发率，这也造成了沉重的经济和社会负担。在这里，我们开发了一种由阿仑膦酸钠（AS）/Mg2负载的反蛋白石甲基丙烯化明胶（GelMA）水凝胶微球（IOHM-AS-MGs）在甲基丙烯化聚（透明质酸）（HAMA）中组成的分层水凝胶，用于骨质疏松性骨缺损治疗。IOHM-AS-MGs显示良好的细胞相容性和细胞粘附性，并在转录组和蛋白质水平上强烈刺激成骨。当这种治疗应用于骨质疏松性骨缺损区域时，使用HAMA固定微球。显微计算机断层扫描（micro-CT）和组织学分析的结果表明，分层水凝胶具有最佳的治疗效果。因此,该水凝胶是骨质疏松性骨缺损治疗的新候选物。
