Abstract：
Although cells are frequently maintained at cold temperatures during experiments, the effects of cold stress on cell viability and subsequent cellular conditions remain elusive. In this study, we investigated the effects of cold stress on cancer cells under various culture conditions. We showed that cold stress induces ferroptosis, a form of cell death characterized by lipid peroxidation, in sensitive cancer cell lines. High cell density and serum starvation activate the Hippo pathway and suppress cold-induced cell death. Genetic deletion of Hippo pathway components enhances cold stress susceptibility. Furthermore, the cell attachment status influences the response to cold stress, with suspended cells showing greater resistance and faster recovery than attached cells. This study highlights the importance of cellular conditions and the Hippo pathway in the handling and storage of cancer cells at cold temperatures, thereby offering insights into experimental and clinical contexts.
摘要：
尽管细胞在实验过程中经常保持在低温下，冷应激对细胞活力和随后的细胞条件的影响仍然难以捉摸。在这项研究中,我们研究了不同培养条件下冷应激对癌细胞的影响。我们证明了冷应激诱导铁死亡，一种以脂质过氧化为特征的细胞死亡形式，在敏感的癌细胞系中。高细胞密度和血清饥饿激活Hippo途径并抑制冷诱导的细胞死亡。Hippo途径组分的遗传缺失增强冷应激易感性。此外，细胞附着状态影响对冷应激的反应，悬浮细胞比附着细胞表现出更大的抗性和更快的恢复。这项研究强调了细胞条件和Hippo途径在低温下处理和储存癌细胞的重要性，从而提供对实验和临床背景的见解。
