METHODS: Cell viability, proliferation capacity and yield were assessed. Characterization of stem cell potency was performed by analyzing cell surface markers including CD34, a marker of activated adipose-derived stem cells. The trilineage differentiation potential was evaluated using RT-PCR and histology.
RESULTS: The yield rate of mSVF obtained from MCAM was significantly higher than that with the conventional method, although use of the device resulted in a slight decrease in cell viability. After culture, mASCs exhibited a remarkable clonogenic potential and significantly higher cell proliferation potential than cASCs. The mASCs also displayed a distinct pattern of ASC cell surface markers, increased expression of genes related to CD34, high pluripotency, and a high trilineage differentiation ability.
CONCLUSIONS: The specialized device enhanced the yield of SVF and produced cells with high proliferation rates and characteristics that include expression of stem cell markers.
方法:细胞活力,评估增殖能力和产量。通过分析包括CD34(活化的脂肪来源的干细胞的标志物)的细胞表面标志物进行干细胞效力的表征。使用RT-PCR和组织学评估三系分化潜能。
结果:从MCAM获得的mSVF的产率明显高于常规方法,尽管使用该装置导致细胞活力略有下降。文化之后,mASC表现出显著的克隆形成潜能和显著高于cASC的细胞增殖潜能。mASC还展示了ASC细胞表面标志物的独特模式,与CD34相关的基因表达增加,高多能性,和高三系分化能力。
结论:专用装置提高了SVF的产量,并产生了具有高增殖率和包括干细胞标志物表达的特征的细胞。