Abstract：
BACKGROUND: A specialized device equipped with a sharp blade filter has been developed to enable more efficient purification of a micronized cellular adipose matrix (MCAM) containing stem cells. The aim of this study is to compare the characteristics and functions of the population of stromal cells (mSVF) and cultured cells (mASCs) purified using this device with those of cSVF and cASCs obtained through conventional enzymatic purification.
METHODS: Cell viability, proliferation capacity and yield were assessed. Characterization of stem cell potency was performed by analyzing cell surface markers including CD34, a marker of activated adipose-derived stem cells. The trilineage differentiation potential was evaluated using RT-PCR and histology.
RESULTS: The yield rate of mSVF obtained from MCAM was significantly higher than that with the conventional method, although use of the device resulted in a slight decrease in cell viability. After culture, mASCs exhibited a remarkable clonogenic potential and significantly higher cell proliferation potential than cASCs. The mASCs also displayed a distinct pattern of ASC cell surface markers, increased expression of genes related to CD34, high pluripotency, and a high trilineage differentiation ability.
CONCLUSIONS: The specialized device enhanced the yield of SVF and produced cells with high proliferation rates and characteristics that include expression of stem cell markers.
METHODS: Cell viability, proliferation capacity and yield were assessed. Characterization of stem cell potency was performed by analyzing cell surface markers including CD34, a marker of activated adipose-derived stem cells. The trilineage differentiation potential was evaluated using RT-PCR and histology.
RESULTS: The yield rate of mSVF obtained from MCAM was significantly higher than that with the conventional method, although use of the device resulted in a slight decrease in cell viability. After culture, mASCs exhibited a remarkable clonogenic potential and significantly higher cell proliferation potential than cASCs. The mASCs also displayed a distinct pattern of ASC cell surface markers, increased expression of genes related to CD34, high pluripotency, and a high trilineage differentiation ability.
CONCLUSIONS: The specialized device enhanced the yield of SVF and produced cells with high proliferation rates and characteristics that include expression of stem cell markers.
摘要：
背景：已经开发了配备有锋利刀片过滤器的专用设备，以能够更有效地纯化包含干细胞的微粉化细胞脂肪基质（MCAM）。这项研究的目的是比较使用该设备纯化的基质细胞（mSVF）和培养细胞（mASCs）与通过常规酶促纯化获得的cSVF和cASCs的特征和功能。
方法：细胞活力，评估增殖能力和产量。通过分析包括CD34(活化的脂肪来源的干细胞的标志物)的细胞表面标志物进行干细胞效力的表征。使用RT-PCR和组织学评估三系分化潜能。
结果：从MCAM获得的mSVF的产率明显高于常规方法，尽管使用该装置导致细胞活力略有下降。文化之后，mASC表现出显著的克隆形成潜能和显著高于cASC的细胞增殖潜能。mASC还展示了ASC细胞表面标志物的独特模式，与CD34相关的基因表达增加，高多能性，和高三系分化能力。
结论：专用装置提高了SVF的产量，并产生了具有高增殖率和包括干细胞标志物表达的特征的细胞。
方法：细胞活力，评估增殖能力和产量。通过分析包括CD34(活化的脂肪来源的干细胞的标志物)的细胞表面标志物进行干细胞效力的表征。使用RT-PCR和组织学评估三系分化潜能。
结果：从MCAM获得的mSVF的产率明显高于常规方法，尽管使用该装置导致细胞活力略有下降。文化之后，mASC表现出显著的克隆形成潜能和显著高于cASC的细胞增殖潜能。mASC还展示了ASC细胞表面标志物的独特模式，与CD34相关的基因表达增加，高多能性，和高三系分化能力。
结论：专用装置提高了SVF的产量，并产生了具有高增殖率和包括干细胞标志物表达的特征的细胞。
