Abstract：
In this work, a near-infrared hepatocyte-targeting fluorescence probe TCF-Gal-Cys was developed. The TCF-Gal-Cys exhibited a low detection limit, good sensitivity and selectivity toward Cys. The responsive mechanism of TCF-Gal-Cys was proposed that the acrylate of TCF-Gal-Cys was subsequently attacked by the thiol group and the amino group of Cys, releasing a strong near-infrared fluorescent group. TCF-Gal-Cys displayed a good hepatocyte-targeting capacity and could specifically distinguish hepatocytes from A549, Hela, SGC-7901 cells because the galactose group of TCF-Gal-Cys can be recognized by HepG2 cells overexpressing ASGPR. The TCF-Gal-Cys has achieved excellently imaging performance to Cys in the zebrafish, so TCF-Gal-Cys has potential to be an effective tool to in real time monitor Cys-related diseases in vitro and in vivo.
摘要：
在这项工作中,开发了近红外肝细胞靶向荧光探针TCF-Gal-Cys。TCF-Gal-Cys表现出低检测限，对Cys具有良好的灵敏度和选择性。提出了TCF-Gal-Cys的响应机制，即TCF-Gal-Cys的丙烯酸酯随后受到Cys的巯基和氨基的攻击，释放出强烈的近红外荧光基团。TCF-Gal-Cys表现出良好的肝细胞靶向能力，可以特异性区分A549，Hela，SGC-7901细胞因为TCF-Gal-Cys的半乳糖基团可被过表达ASGPR的HepG2细胞所辨认。TCF-Gal-Cys在斑马鱼中对Cys具有优异的成像性能，因此TCF-Gal-Cys有可能成为体内外实时监测Cys相关疾病的有效工具。
