Abstract：
Dopamine is an important neurotransmitter in the body and closely related to many neurodegenerative diseases. Therefore, the detection of dopamine is of great significance for the diagnosis and treatment of diseases, screening of drugs and unraveling of relevant pathogenic mechanisms. However, the low concentration of dopamine in the body and the complexity of the matrix make the accurate detection of dopamine challenging. Herein, an electrochemical sensor is constructed based on ternary nanocomposites consisting of one-dimensional Pt nanowires, two-dimensional MXene nanosheets, and three-dimensional porous carbon. The Pt nanowires exhibit excellent catalytic activity due to the abundant grain boundaries and highly undercoordinated atoms; MXene nanosheets not only facilitate the growth of Pt nanowires, but also enhance the electrical conductivity and hydrophilicity; and the porous carbon helps induce significant adsorption of dopamine on the electrode surface. In electrochemical tests, the ternary nanocomposite-based sensor achieves an ultra-sensitive detection of dopamine (S/N = 3) with a low limit of detection (LOD) of 28 nM, satisfactory selectivity and excellent stability. Furthermore, the sensor can be used for the detection of dopamine in serum and in situ monitoring of dopamine release from PC12 cells. Such a highly sensitive nanocomposite sensor can be exploited for in situ monitoring of important neurotransmitters at the cellular level, which is of great significance for related drug screening and mechanistic studies.
摘要：
多巴胺是体内重要的神经递质,与许多神经退行性疾病密切相关。因此,多巴胺的检测对疾病的诊断和治疗具有重要意义,药物筛选和相关致病机制的揭示。然而,多巴胺在体内的浓度低和基质的复杂性使得多巴胺的准确检测具有挑战性。在这里，基于由一维Pt纳米线组成的三元纳米复合材料构建电化学传感器,二维MXene纳米片，和三维多孔碳。由于丰富的晶界和高度欠配位的原子，Pt纳米线表现出优异的催化活性；MXene纳米片不仅有利于Pt纳米线的生长，而且还增强了导电性和亲水性；多孔碳有助于诱导多巴胺在电极表面的显著吸附。在电化学测试中，基于三元纳米复合材料的传感器实现了多巴胺（S/N=3）的超灵敏检测，低检测限（LOD）为28nM，令人满意的选择性和优异的稳定性。此外，该传感器可用于血清中多巴胺的检测和PC12细胞多巴胺释放的原位监测。这种高度敏感的纳米复合材料传感器可以用于在细胞水平上原位监测重要的神经递质，对相关药物筛选和机理研究具有重要意义。
