Abstract：
3D printing can revolutionize personalized medicine by allowing cost-effective, customized tissue-engineering constructs. However, the limited availability and diversity of biopolymeric hydrogels restrict the variety and applications of bioinks. In this study, we introduce a composite bioink for 3D bioprinting, combining a photo-cross-linkable derivative of Mucin (Mu) called Methacrylated Mucin (MuMA) and Hyaluronic acid (HA). The less explored Mucin is responsible for the hydrogel nature of mucus and holds the potential to be used as a bioink material because of its plethora of features. HA, a crucial extracellular matrix component, is mucoadhesive and enhances ink viscosity and printability. Photo-cross-linking with 405 nm light stabilizes the printed scaffolds without damaging cells. Rheological tests reveal shear-thinning behavior, aiding cell protection during printing and improved MuMA bioink viscosity by adding HA. The printed structures exhibited porous behavior conducive to nutrient transport and cell migration. After 4 weeks in phosphate-buffered saline, the scaffolds retain 70% of their mass, highlighting stability. Biocompatibility tests with lung epithelial cells (L-132) confirm cell attachment and growth, suggesting suitability for lung tissue engineering. It is envisioned that the versatility of bioink could lead to significant advancements in lung tissue engineering and various other biomedical applications.
摘要：
3D打印可以通过允许具有成本效益的个性化医疗彻底改变，定制的组织工程构造。然而,生物聚合水凝胶的有限可用性和多样性限制了生物墨水的种类和应用。在这项研究中,我们引入了一种用于3D生物打印的复合生物墨水，结合称为甲基丙烯酸酯化粘蛋白(MuMA)的粘蛋白(Mu)的光交联衍生物和透明质酸(HA)。较少探索的粘蛋白负责粘液的水凝胶性质，并且由于其丰富的特征而具有用作生物墨水材料的潜力。HA,一种重要的细胞外基质成分，是粘膜粘附性和增强油墨粘度和可印刷性。用405nm光的光交联使打印的支架稳定而不损伤细胞。流变试验揭示了剪切稀化行为，通过添加HA来帮助打印过程中的细胞保护并改善MuMA生物墨水粘度。打印结构表现出有利于养分运输和细胞迁移的多孔行为。在磷酸盐缓冲盐水中4周后，支架保留了70%的质量，强调稳定性。与肺上皮细胞（L-132）的生物相容性试验证实细胞附着和生长，表明肺组织工程的适用性。可以预见，生物墨水的多功能性可能会导致肺组织工程和各种其他生物医学应用的显着进步。
