PDF(Pubmed)
Abstract：
Astaxanthin is a powerful antioxidant known to enhance skin, cardiovascular, eye, and brain health. In this study, the genome insights and astaxanthin production of two newly isolated astaxanthin-producing yeasts (TL35-5 and PL61-2) were evaluated and compared. Based on their phenotypic and genotypic characteristics, TL35-5 and PL61-2 were identified as basidiomycetous yeasts belonging to Rhodotorula paludigena and Rhodotorula sampaioana, respectively. To optimize astaxanthin production, the effects of cultural medium composition and cultivation conditions were examined. The optimal conditions for astaxanthin production in R. paludigena TL35-5 involved cultivation in AP medium containing 10 g/L glucose as the sole carbon source, supplemented with 1.92 g/L potassium nitrate, pH 6.5, and incubation at 20°C for 3 days with shaking at 200 rpm. For R. sampaioana PL61-2, the optimal medium composition for astaxanthin production consisted of AP medium with 40 g/L glucose, supplemented with 0.67 g/L urea, pH 7.5, and the fermentation was carried out at 20°C for 3 days with agitating at 200 rpm. Under their optimal conditions, R. paludigena TL35-5 and R. sampaioana PL61-2 gave the highest astaxanthin yields of 3.689 ± 0.031 and 4.680 ± 0.019 mg/L, respectively. The genome of TL35-5 was 20,982,417 bp in length, with a GC content of 64.20%. A total of 6,789 protein-encoding genes were predicted. Similarly, the genome of PL61-2 was 21,374,169 bp long, with a GC content of 64.88%. It contained 6,802 predicted protein-encoding genes. Furthermore, all essential genes involved in astaxanthin biosynthesis, including CrtE, CrtYB, CrtI, CrtS, and CrtR, were identified in both R. paludigena TL35-5 and R. sampaioana PL61-2, providing evidence for their ability to produce astaxanthin.
摘要：
虾青素是一种强大的抗氧化剂，可以增强皮肤，心血管,眼睛,和大脑健康。在这项研究中,评估并比较了两种新分离的虾青素产生酵母(TL35-5和PL61-2)的基因组见解和虾青素产生。根据他们的表型和基因型特征，TL35-5和PL61-2被鉴定为担子菌酵母，属于红斑红和红斑红，分别。优化虾青素生产，研究了培养基成分和栽培条件的影响。在R.paludigenaTL35-5中生产虾青素的最佳条件涉及在含有10g/L葡萄糖作为唯一碳源的AP培养基中培养，补充1.92g/L硝酸钾，pH6.5,并在200rpm振荡下在20°C下孵育3天。对于R.sampaioanaPL61-2，虾青素生产的最佳培养基组成由含有40g/L葡萄糖的AP培养基组成，补充0.67g/L尿素，pH7.5,发酵在20°C下进行3天,同时以200rpm搅拌。在他们的最佳条件下，R.paludigenaTL35-5和R.sampaioanaPL61-2的虾青素产量最高，分别为3.689±0.031和4.680±0.019mg/L，分别。TL35-5基因组长度为20,982,417bp,GC含量为64.20%。预测了总共6,789个蛋白质编码基因。同样，PL61-2的基因组长21,374,169bp,GC含量为64.88%。它包含6,802个预测的蛋白质编码基因。此外，所有参与虾青素生物合成的必需基因，包括CrtE,CrtYB,CrtI,CrtS,和CrtR,在R.paludigenaTL35-5和R.sampaioanaPL61-2中都鉴定出，为它们产生虾青素的能力提供了证据。
