Abstract：
Hydatid cyst is the metacestode stage of Echinococcus granulosus that occurs in herbivores and humans as intermediate hosts by consuming parasite eggs through forage and vegetables. Carnivores, as definitive hosts, become infected by consuming infected vesicles of herbivores. The most effective treatment for a hydatid cyst is surgical operation. Inactivating E. granulosus protoscoleces through heating, cooling, or chemicals such as sodium chloride can be considered an effective method for controlling hydatidosis in both humans and animals. The main objective of this study was to evaluate the effect of different temperatures and salinity conditions on the survival of Echinococcus granulosus protoscoleces. For this purpose, 50 g of infected liver (in triplicate) was separately treated with different temperatures (+10°C, +50°C, +60°C, +72°C, and -20°C) and concentrations of sodium chloride (5%, 10%, 15%, and 20%) for 3, 6, 12, 24, 48, and 72 h. Additionally, 50 g of infected liver was stored separately in the refrigerator (+4°C) as a control group. The survival rate of the protoscoleces was evaluated by staining with 1% eosin under a light microscope. The results showed that the protoscoleces were significantly affected, with 100% mortality at -20°C after 0.5 h, and complete death at +72°C, +60°C, +50°C, and +10°C after 1, 1.5, 3, and 24 h, respectively (p < 0.005). Similarly, the protoscoleces in the liver mass survived at 5% NaCl after 3 h but died at 10% after 24 h, at 15% after 12 h, and at 20% after 6 h. It is concluded that exposing the liver infected with protoscoleces hydatid cyst to a temperature of -20°C and a sodium chloride concentration of 10% for 24 h is suitable for inactivating the protoscoleces.
摘要：
包虫囊肿是细粒棘球蚴的后生阶段，发生在草食动物和人类作为中间宿主，通过饲草和蔬菜消耗寄生虫卵。食肉动物,作为最终的主人，通过消耗被感染的食草动物囊泡而被感染。包虫囊肿最有效的治疗方法是外科手术。通过加热灭活E.granulosus原型，冷却,或化学品如氯化钠可被认为是控制人和动物葡萄胎病的有效方法。本研究的主要目的是评估不同温度和盐度条件对细粒棘球蚴原头节存活的影响。为此,分别用不同温度（+10°C，+50°C,+60°C,+72°C,和-20°C）和氯化钠浓度（5%，10%,15%,和20%)持续3、6、12、24、48和72小时。此外，将50g感染的肝脏单独储存在冰箱(+4°C)中作为对照组。通过在光学显微镜下用1％伊红染色来评估原头节的存活率。结果表明，原头肌受到显著影响，0.5h后在-20°C下死亡率为100%，在+72°C时完全死亡，+60°C,+50°C,1、1.5、3和24小时后+10°C，分别为(p<0.005)。同样，在3小时后，在5％NaCl下，肝脏块中的原节存活，但在24小时后死亡10％，12小时后15%，6h后为20％。结论是，将感染了原头囊包虫囊肿的肝脏暴露于-20℃的温度和10％的氯化钠浓度下24h适合于灭活原头囊。
