Abstract：
Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzicola (Xoc), is a major bacterial disease in rice. Transcription activator-like effectors (TALEs) from Xanthomonas can induce host susceptibility (S) genes and facilitate infection. However, knowledge of the function of Xoc TALEs in promoting bacterial virulence is limited. In this study, we demonstrated the importance of Tal10a for the full virulence of Xoc. Through computational prediction and gene expression analysis, we identified the hexokinase gene OsHXK5 as a host target of Tal10a. Tal10a directly binds to the gene promoter region and activates the expression of OsHXK5. CRISPR/Cas9-mediated gene editing in the effector binding element (EBE) of OsHXK5 significantly increases rice resistance to Xoc, while OsHXK5 overexpression enhances the susceptibility of rice plants and impairs rice defense responses. Moreover, simultaneous editing of the promoters of OsSULTR3;6 and OsHXK5 confers robust resistance to Xoc in rice. Taken together, our findings highlight the role of Tal10a in targeting OsHXK5 to promote infection and suggest that OsHXK5 represents a potential target for engineering rice resistance to Xoc.
摘要：
细菌条纹（BLS），由米黄单胞菌pv引起。oryzicola(Xoc),是水稻的主要细菌性病害。黄单胞菌的转录激活因子样效应子（TALE）可以诱导宿主敏感性（S）基因并促进感染。然而,对XocTALE在促进细菌毒力方面的功能的了解是有限的。在这项研究中,我们证明了Tal10a对Xoc全毒力的重要性。通过计算预测和基因表达分析,我们确定己糖激酶基因OsHXK5为Tal10a的宿主靶标。Tal10a直接结合基因启动子区并激活OsHXK5的表达。在OsHXK5的效应子结合元件(EBE)中CRISPR/Cas9介导的基因编辑显著增加了水稻对Xoc的抗性,而OsHXK5过表达增强了水稻植株的易感性并损害了水稻防御反应。此外，同时编辑OsSULTR3的启动子;6和OsHXK5赋予水稻对Xoc的强烈抗性。一起来看,我们的发现强调了Tal10a在靶向OsHXK5促进感染中的作用，并表明OsHXK5是工程水稻对Xoc抗性的潜在靶标。
