PDF(Pubmed)
Abstract：
CONCLUSIONS: Reactive nitrogen species mitigate the deteriorative effect of accelerated seed ageing by affecting the glutathione concentration and activities of GR and GPX-like. The treatment of apple (Malus domestica Borkh.) embryos isolated from accelerated aged seeds with nitric oxide-derived compounds increases their vigour and is linked to the alleviation of the negative effect of excessive oxidation processes. Reduced form of glutathione (GSH) is involved in the maintenance of redox potential. Glutathione peroxidase-like (GPX-like) uses GSH and converts it to oxidised form (GSSG), while glutathione reductase (GR) reduces GSSG into GSH. The aim of this work was to investigate the impact of the short-time NOx treatment of embryos isolated from apple seeds subjected to accelerated ageing on glutathione-related parameters. Apple seeds were subjected to accelerated ageing for 7, 14 or 21 days. Isolated embryos were shortly treated with NOx and cultured for 48 h. During ageing, in the axes of apple embryos, GSH and GSSG levels as well as half-cell reduction potential remained stable, while GR and GPX-like activities decreased. However, the positive effect of NOx in the vigour preservation of embryos isolated from prolonged aged seeds is linked to the increased total glutathione pool, and above all, higher GSH content. Moreover, NOx increased the level of transcripts encoding GPX-like and stimulated enzymatic activity. The obtained results indicate that high seed vigour related to the mode of action of NO and its derivatives is closely linked to the maintenance of higher GSH levels.
摘要：
结论：活性氮物种通过影响谷胱甘肽浓度以及GR和GPX样的活性来减轻加速种子老化的退化作用。苹果（MalusdomesticaBorkh。）用一氧化氮衍生的化合物从加速老化的种子中分离出的胚胎增加了它们的活力，并与减轻过度氧化过程的负面影响有关。还原型谷胱甘肽(GSH)参与氧化还原电位的维持。谷胱甘肽过氧化物酶样（GPX样）使用GSH并将其转化为氧化形式（GSSG），而谷胱甘肽还原酶（GR）将GSSG还原为GSH。这项工作的目的是研究从加速老化的苹果种子中分离出的胚胎的短时NOx处理对谷胱甘肽相关参数的影响。使苹果种子加速老化7、14或21天。将分离的胚胎用NOx短暂处理并培养48小时。在老化期间，在苹果胚胎的轴上，GSH和GSSG水平以及半细胞还原电位保持稳定，而GR和GPX样活性下降。然而,NOx在保存从长期老化种子中分离的胚胎的活力中的积极作用与总谷胱甘肽池的增加有关，最重要的是,GSH含量较高。此外，NOx增加了编码GPX样和刺激酶活性的转录物的水平。获得的结果表明，与NO及其衍生物的作用方式有关的高种子活力与维持较高的GSH水平密切相关。
