UNASSIGNED: Phytochemical screening was performed using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity was assessed against Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhi, Staphylococcus aureus, Listeria monocytogenes, and Bacillus subtilis using an agar diffusion test with the determination of the minimal inhibition concentration (MIC), while the antioxidant activity was determined using different chemical methods (DPPH, ABTS, CUPRAC, reducing power, superoxide anion scavenging, β-carotene bleaching, and metal chelate). In addition, cytotoxic activity was tested using Artemia salina (Brine shrimp) bioassay.
UNASSIGNED: The studied extract exhibited intense antibacterial activity against E. coli and S. aureus with inhibition diameters of 28 ± 0.01 and 22 ± 0.01 mm, respectively, with a MIC value of 6.25 mg/mL and a selectivity index of 2.8. The obtained extract showed different antioxidant trends depending on the selected assay. GC-MS analysis revealed many secondary metabolites.
UNASSIGNED: P. venusta, a type of lichen, is a potential source of bioactive substances that could be used in pharmaceuticals.
使用气相色谱-质谱(GC-MS)进行植物化学筛选。对大肠杆菌的抗菌活性进行了评估,铜绿假单胞菌,肠炎沙门氏菌,伤寒沙门氏菌,金黄色葡萄球菌,单核细胞增生李斯特菌,和枯草芽孢杆菌使用琼脂扩散试验,测定最小抑制浓度(MIC),而抗氧化活性是使用不同的化学方法(DPPH,ABTS,CUPRAC,降低功率,清除超氧阴离子,β-胡萝卜素漂白,和金属螯合物)。此外,使用卤虫(盐水虾)生物测定法测试细胞毒性活性。
■研究的提取物对大肠杆菌和金黄色葡萄球菌具有强烈的抗菌活性,抑制直径为28±0.01和22±0.01mm,分别,MIC值为6.25mg/mL,选择性指数为2.8。所获得的提取物显示不同的抗氧化趋势,这取决于所选择的测定。GC-MS分析显示许多次生代谢物。
■P.维努斯塔,一种地衣,是可用于药物的生物活性物质的潜在来源。