PDF(Pubmed)
Abstract：
UNASSIGNED: Maternal intervillous monocytes (MIMs) and fetal Hofbauer cells (HBCs) are myeloid-derived immune cells at the maternal-fetal interface. Maternal reproductive history is associated with differential risk of pregnancy complications. The molecular phenotypes and roles of these distinct monocyte/macrophage populations and the influence of gravidity on these phenotypes has not been systematically investigated.
UNASSIGNED: Here, we used RNA sequencing to study the transcriptional profiles of MIMs and HBCs in normal term pregnancies.
UNASSIGNED: Our analyses revealed distinct transcriptomes of MIMs and HBCs. Genes involved in differentiation and cell organization pathways were more highly expressed in MIMs vs. HBCs. In contrast, HBCs had higher expression of genes involved in inflammatory responses and cell surface receptor signaling. Maternal gravidity influenced monocyte programming, as expression of pro-inflammatory molecules was significantly higher in MIMs from multigravidae compared to primigravidae. In HBCs, multigravidae displayed enrichment of gene pathways involved in cell-cell signaling and differentiation.
UNASSIGNED: Our results demonstrated that MIMs and HBCs have highly divergent transcriptional signatures, reflecting their distinct origins, locations, functions, and roles in inflammatory responses. Furthermore, maternal gravidity influences the gene signatures of MIMs and HBCs, potentially modulating the interplay between tolerance and trained immunity. The phenomenon of reproductive immune memory may play a novel role in the differential susceptibility of primigravidae to pregnancy complications.
UNASSIGNED: Here, we used RNA sequencing to study the transcriptional profiles of MIMs and HBCs in normal term pregnancies.
UNASSIGNED: Our analyses revealed distinct transcriptomes of MIMs and HBCs. Genes involved in differentiation and cell organization pathways were more highly expressed in MIMs vs. HBCs. In contrast, HBCs had higher expression of genes involved in inflammatory responses and cell surface receptor signaling. Maternal gravidity influenced monocyte programming, as expression of pro-inflammatory molecules was significantly higher in MIMs from multigravidae compared to primigravidae. In HBCs, multigravidae displayed enrichment of gene pathways involved in cell-cell signaling and differentiation.
UNASSIGNED: Our results demonstrated that MIMs and HBCs have highly divergent transcriptional signatures, reflecting their distinct origins, locations, functions, and roles in inflammatory responses. Furthermore, maternal gravidity influences the gene signatures of MIMs and HBCs, potentially modulating the interplay between tolerance and trained immunity. The phenomenon of reproductive immune memory may play a novel role in the differential susceptibility of primigravidae to pregnancy complications.
摘要：
母体绒毛间单核细胞(MIMs)和胎儿Hofbauer细胞(HBC)是母体-胎儿界面处的骨髓来源的免疫细胞。产妇生育史与妊娠并发症的不同风险相关。尚未系统地研究这些独特的单核细胞/巨噬细胞群体的分子表型和作用以及重力对这些表型的影响。
■这里，我们使用RNA测序来研究正常足月妊娠中MIMs和HBCs的转录谱.
■我们的分析揭示了MIMs和HBCs的不同转录组。参与分化和细胞组织途径的基因在MIMs中表达更高HBCs。相比之下,HBCs具有与炎症反应和细胞表面受体信号传导有关的基因的较高表达。母体妊娠影响单核细胞编程，由于促炎分子在多胎科MIMs中的表达明显高于初胎科MIMs。在HBCs中，多胎科表现出参与细胞-细胞信号传导和分化的基因途径的富集。
■我们的结果表明，MIMs和HBC具有高度不同的转录特征，反映了它们不同的起源，地点,功能,以及在炎症反应中的作用。此外，母体妊娠会影响MIMs和HBCs的基因特征，潜在地调节耐受性和训练免疫力之间的相互作用。生殖免疫记忆现象可能在primigravidae对妊娠并发症的不同易感性中起新作用。
■这里，我们使用RNA测序来研究正常足月妊娠中MIMs和HBCs的转录谱.
■我们的分析揭示了MIMs和HBCs的不同转录组。参与分化和细胞组织途径的基因在MIMs中表达更高HBCs。相比之下,HBCs具有与炎症反应和细胞表面受体信号传导有关的基因的较高表达。母体妊娠影响单核细胞编程，由于促炎分子在多胎科MIMs中的表达明显高于初胎科MIMs。在HBCs中，多胎科表现出参与细胞-细胞信号传导和分化的基因途径的富集。
■我们的结果表明，MIMs和HBC具有高度不同的转录特征，反映了它们不同的起源，地点,功能,以及在炎症反应中的作用。此外，母体妊娠会影响MIMs和HBCs的基因特征，潜在地调节耐受性和训练免疫力之间的相互作用。生殖免疫记忆现象可能在primigravidae对妊娠并发症的不同易感性中起新作用。
